7M0R

Cryo-EM structure of the Sema3A/PlexinA4/Neuropilin 1 complex

7M0R の概要

• エントリーDOI: 10.2210/pdb7m0r/pdb
• EMDBエントリー: 23613
• 分子名称: Neuropilin-1, Semaphorin-3A, Plexin-A4, ... (4 entities in total)
• 機能のキーワード: plexin, semaphorin, neuropilin, signaling, signaling protein
• 由来する生物種: Mus musculus (Mouse); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 530794.76

構造登録者

Lu, D.,Shang, G.,He, X.,Bai, X.,Zhang, X. (登録日: 2021-03-11, 公開日: 2021-05-05, 最終更新日: 2024-10-16)

主引用文献

Lu, D.,Shang, G.,He, X.,Bai, X.C.,Zhang, X.
Architecture of the Sema3A/PlexinA4/Neuropilin tripartite complex.
Nat Commun, 12:3172-3172, 2021

PubMed Abstract: Secreted class 3 semaphorins (Sema3s) form tripartite complexes with the plexin receptor and neuropilin coreceptor, which are both transmembrane proteins that together mediate semaphorin signal for neuronal axon guidance and other processes. Despite extensive investigations, the overall architecture of and the molecular interactions in the Sema3/plexin/neuropilin complex are incompletely understood. Here we present the cryo-EM structure of a near intact extracellular region complex of Sema3A, PlexinA4 and Neuropilin 1 (Nrp1) at 3.7 Å resolution. The structure shows a large symmetric 2:2:2 assembly in which each subunit makes multiple interactions with others. The two PlexinA4 molecules in the complex do not interact directly, but their membrane proximal regions are close to each other and poised to promote the formation of the intracellular active dimer for signaling. The structure reveals a previously unknown interface between the a2b1b2 module in Nrp1 and the Sema domain of Sema3A. This interaction places the a2b1b2 module at the top of the complex, far away from the plasma membrane where the transmembrane regions of Nrp1 and PlexinA4 embed. As a result, the region following the a2b1b2 module in Nrp1 must span a large distance to allow the connection to the transmembrane region, suggesting an essential role for the long non-conserved linkers and the MAM domain in neuropilin in the semaphorin/plexin/neuropilin complex.

PubMed: 34039996
DOI: 10.1038/s41467-021-23541-x

実験手法

ELECTRON MICROSCOPY (3.7 Å)