+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-23613 | ||||||||||||||||||
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Title | Cryo-EM structure of the Sema3A/PlexinA4/Neuropilin 1 complex | ||||||||||||||||||
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Sample |
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Function / homology | Function and homology information Neurophilin interactions with VEGF and VEGFR / neural crest cell migration involved in sympathetic nervous system development / glossopharyngeal nerve morphogenesis / chemorepulsion of branchiomotor axon / regulation of negative chemotaxis / vagus nerve morphogenesis / cell migration involved in coronary vasculogenesis / anterior commissure morphogenesis / cranial nerve morphogenesis / trigeminal nerve morphogenesis ...Neurophilin interactions with VEGF and VEGFR / neural crest cell migration involved in sympathetic nervous system development / glossopharyngeal nerve morphogenesis / chemorepulsion of branchiomotor axon / regulation of negative chemotaxis / vagus nerve morphogenesis / cell migration involved in coronary vasculogenesis / anterior commissure morphogenesis / cranial nerve morphogenesis / trigeminal nerve morphogenesis / Signal transduction by L1 / regulation of axon extension involved in axon guidance / postganglionic parasympathetic fiber development / synaptic target recognition / facial nerve morphogenesis / sympathetic neuron axon guidance / CRMPs in Sema3A signaling / basal dendrite development / protein localization to early endosome / otic placode development / neurofilament / basal dendrite arborization / Sema3A PAK dependent Axon repulsion / dichotomous subdivision of terminal units involved in salivary gland branching / retina vasculature morphogenesis in camera-type eye / regulation of vascular endothelial growth factor receptor signaling pathway / vestibulocochlear nerve structural organization / semaphorin receptor binding / dorsal root ganglion morphogenesis / epithelial cell migration / ventral trunk neural crest cell migration / sympathetic neuron projection guidance / facioacoustic ganglion development / trigeminal ganglion development / cerebellar climbing fiber to Purkinje cell synapse / trigeminal nerve structural organization / sensory neuron axon guidance / facial nerve structural organization / gonadotrophin-releasing hormone neuronal migration to the hypothalamus / postsynapse organization / branchiomotor neuron axon guidance / renal artery morphogenesis / positive regulation of male gonad development / SEMA3A-Plexin repulsion signaling by inhibiting Integrin adhesion / semaphorin-plexin signaling pathway involved in axon guidance / maintenance of synapse structure / axon extension involved in axon guidance / VEGF-activated neuropilin signaling pathway / negative regulation of axon extension involved in axon guidance / retina vasculature development in camera-type eye / blood vessel endothelial cell migration / sympathetic neuron projection extension / negative regulation of epithelial cell migration / motor neuron migration / endothelial cell chemotaxis / vascular endothelial growth factor binding / sympathetic ganglion development / axonogenesis involved in innervation / neural crest cell migration involved in autonomic nervous system development / positive regulation of axon extension involved in axon guidance / negative regulation of axon extension / vascular endothelial growth factor receptor activity / substrate-dependent cell migration, cell extension / nerve development / semaphorin receptor complex / positive regulation of neuron migration / angiogenesis involved in coronary vascular morphogenesis / olfactory bulb development / sympathetic nervous system development / hepatocyte growth factor receptor signaling pathway / neuropilin signaling pathway / neuropilin binding / coronary artery morphogenesis / outflow tract septum morphogenesis / regulation of Cdc42 protein signal transduction / chemorepellent activity / semaphorin receptor activity / commissural neuron axon guidance / embryonic heart tube development / negative regulation of cell adhesion / axon extension / motor neuron axon guidance / cell migration involved in sprouting angiogenesis / retinal ganglion cell axon guidance / artery morphogenesis / axonal fasciculation / dendrite morphogenesis / sprouting angiogenesis / neural crest cell migration / positive regulation of filopodium assembly / negative chemotaxis / branching involved in blood vessel morphogenesis / cellular response to hepatocyte growth factor stimulus / positive regulation of axonogenesis / positive regulation of cell migration involved in sprouting angiogenesis / positive chemotaxis / dendrite development / growth factor binding / sorting endosome / positive regulation of focal adhesion assembly Similarity search - Function | ||||||||||||||||||
Biological species | Mus musculus (house mouse) | ||||||||||||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.7 Å | ||||||||||||||||||
Authors | Lu D / Shang G / He X / Bai X / Zhang X | ||||||||||||||||||
Funding support | United States, 5 items
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Citation | Journal: Nat Commun / Year: 2021 Title: Architecture of the Sema3A/PlexinA4/Neuropilin tripartite complex. Authors: Defen Lu / Guijun Shang / Xiaojing He / Xiao-Chen Bai / Xuewu Zhang / Abstract: Secreted class 3 semaphorins (Sema3s) form tripartite complexes with the plexin receptor and neuropilin coreceptor, which are both transmembrane proteins that together mediate semaphorin signal for ...Secreted class 3 semaphorins (Sema3s) form tripartite complexes with the plexin receptor and neuropilin coreceptor, which are both transmembrane proteins that together mediate semaphorin signal for neuronal axon guidance and other processes. Despite extensive investigations, the overall architecture of and the molecular interactions in the Sema3/plexin/neuropilin complex are incompletely understood. Here we present the cryo-EM structure of a near intact extracellular region complex of Sema3A, PlexinA4 and Neuropilin 1 (Nrp1) at 3.7 Å resolution. The structure shows a large symmetric 2:2:2 assembly in which each subunit makes multiple interactions with others. The two PlexinA4 molecules in the complex do not interact directly, but their membrane proximal regions are close to each other and poised to promote the formation of the intracellular active dimer for signaling. The structure reveals a previously unknown interface between the a2b1b2 module in Nrp1 and the Sema domain of Sema3A. This interaction places the a2b1b2 module at the top of the complex, far away from the plasma membrane where the transmembrane regions of Nrp1 and PlexinA4 embed. As a result, the region following the a2b1b2 module in Nrp1 must span a large distance to allow the connection to the transmembrane region, suggesting an essential role for the long non-conserved linkers and the MAM domain in neuropilin in the semaphorin/plexin/neuropilin complex. | ||||||||||||||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | EM map: SurfViewMolmilJmol/JSmol |
Supplemental images |
-Downloads & links
-EMDB archive
Map data | emd_23613.map.gz | 75.7 MB | EMDB map data format | |
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Header (meta data) | emd-23613-v30.xml emd-23613.xml | 16.1 KB 16.1 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_23613_fsc.xml | 10 KB | Display | FSC data file |
Images | emd_23613.png | 62 KB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-23613 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-23613 | HTTPS FTP |
-Related structure data
Related structure data | 7m0rMC M: atomic model generated by this map C: citing same article (ref.) |
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Similar structure data |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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Related items in Molecule of the Month |
-Map
File | Download / File: emd_23613.map.gz / Format: CCP4 / Size: 83.7 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Voxel size | X=Y=Z: 1.08 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
-Sample components
-Entire : Ternary complex of Sema3A, PlexinA4 and neuropilin 1
Entire | Name: Ternary complex of Sema3A, PlexinA4 and neuropilin 1 |
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Components |
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-Supramolecule #1: Ternary complex of Sema3A, PlexinA4 and neuropilin 1
Supramolecule | Name: Ternary complex of Sema3A, PlexinA4 and neuropilin 1 / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#3 |
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Source (natural) | Organism: Mus musculus (house mouse) |
Recombinant expression | Organism: Homo sapiens (human) |
Molecular weight | Experimental: 600 kDa/nm |
-Macromolecule #1: Neuropilin-1
Macromolecule | Name: Neuropilin-1 / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO |
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Source (natural) | Organism: Mus musculus (house mouse) |
Molecular weight | Theoretical: 64.132211 KDa |
Recombinant expression | Organism: Homo sapiens (human) |
Sequence | String: FRSDKCGGTI KIENPGYLTS PGYPHSYHPS EKCEWLIQAP EPYQRIMINF NPHFDLEDRD CKYDYVEVID GENEGGRLWG KFCGKIAPS PVVSSGPFLF IKFVSDYETH GAGFSIRYEI FKRGPECSQN YTAPTGVIKS PGFPEKYPNS LECTYIIFAP K MSEIILEF ...String: FRSDKCGGTI KIENPGYLTS PGYPHSYHPS EKCEWLIQAP EPYQRIMINF NPHFDLEDRD CKYDYVEVID GENEGGRLWG KFCGKIAPS PVVSSGPFLF IKFVSDYETH GAGFSIRYEI FKRGPECSQN YTAPTGVIKS PGFPEKYPNS LECTYIIFAP K MSEIILEF ESFDLEQDSN PPGGMFCRYD RLEIWDGFPE VGPHIGRYCG QKTPGRIRSS SGVLSMVFYT DSAIAKEGFS AN YSVLQSS ISEDFKCMEA LGMESGEIHS DQITASSQYG TNWSVERSRL NYPENGWTPG EDSYKEWIQV DLGLLRFVTA VGT QGAISK ETKKKYYVKT YRVDISSNGE DWISLKEGNK AIIFQGNTNP TDVVLGVFSK PLITRFVRIK PVSWETGISM RFEV YGCKI TDYPCSGMLG MVSGLISDSQ ITASNQADRN WMPENIRLVT SRTGWALPPS PHPYTNEWLQ VDLGDEKIVR GVIIQ GGKH RENKVFMRKF KIAYSNNGSD WKTIMDDSKR KAKSFEGNNN YDTPELRTFS PLSTRFIRIY PERATHSGLG LRMELL GCE VEAP |
-Macromolecule #2: Semaphorin-3A
Macromolecule | Name: Semaphorin-3A / type: protein_or_peptide / ID: 2 / Number of copies: 2 / Enantiomer: LEVO |
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Source (natural) | Organism: Mus musculus (house mouse) |
Molecular weight | Theoretical: 67.931812 KDa |
Recombinant expression | Organism: Homo sapiens (human) |
Sequence | String: NYANGKNNVP RLKLSYKEML ESNNVITFNG LANSSSYHTF LLDEERSRLY VGAKDHIFSF NLVNIKDFQK IVWPVSYTRR DECKWKGKD ILKECANFIK VLEAYNQTHL YACGTGAFHP ICTYIEVGHH PEDNIFKLQD SHFENGRGKS PYDPKLLTAS L LIDGELYS ...String: NYANGKNNVP RLKLSYKEML ESNNVITFNG LANSSSYHTF LLDEERSRLY VGAKDHIFSF NLVNIKDFQK IVWPVSYTRR DECKWKGKD ILKECANFIK VLEAYNQTHL YACGTGAFHP ICTYIEVGHH PEDNIFKLQD SHFENGRGKS PYDPKLLTAS L LIDGELYS GTAADFMGRD FAIFRTLGHH HPIRTEQHDS RWLNDPRFIS AHLIPESDNP EDDKVYFFFR ENAIDGEHSG KA THARIGQ ICKNDFGGHR SLVNKWTTFL KARLICSVPG PNGIDTHFDE LQDVFLMNSK DPKNPIVYGV FTTSSNIFKG SAV CMYSMS DVRRVFLGPY AHRDGPNYQW VPYQGRVPYP RPGTCPSKTF GGFDSTKDLP DDVITFARSH PAMYNPVFPI NNRP IMIKT DVNYQFTQIV VDRVDAEDGQ YDVMFIGTDV GTVLKVVSVP KETWHDLEEV LLEEMTVFRE PTTISAMELS TKQQQ LYIG STAGVAQLPL HRCDIYGKAC AECCLARDPY CAWDGSSCSR YFPTAKARTR AQDIRNGDPL THCSD(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) |
-Macromolecule #3: Plexin-A4
Macromolecule | Name: Plexin-A4 / type: protein_or_peptide / ID: 3 / Number of copies: 2 / Enantiomer: LEVO |
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Source (natural) | Organism: Mus musculus (house mouse) |
Molecular weight | Theoretical: 133.253203 KDa |
Recombinant expression | Organism: Homo sapiens (human) |
Sequence | String: KPSFVTFRGE PAEGFNHLVV DERTGHIYLG AVNRIYKLSS DLKVLVTHQT GPDEDNPKCY PPRIVQTCNE PLASTNNVNK MLLIDYKEN RLIACGSLYQ GICKLLRLED LFKLGEPFHK KEHYLSGVNE SGSVFGVIVS YSNFDDKLFI ATAVDGKPEY F PTISSRKL ...String: KPSFVTFRGE PAEGFNHLVV DERTGHIYLG AVNRIYKLSS DLKVLVTHQT GPDEDNPKCY PPRIVQTCNE PLASTNNVNK MLLIDYKEN RLIACGSLYQ GICKLLRLED LFKLGEPFHK KEHYLSGVNE SGSVFGVIVS YSNFDDKLFI ATAVDGKPEY F PTISSRKL TKNSEADGMF AYVFHDEFVA SMIKIPSDTF TVIPDFDIYY VYGFSSGNFV YFLTLQPEMV SPPGSTTKEQ VY TSKLVRL CKEDTAFNSY VEVPIGCERN GVEYRLLQAA YLSKAGAVLG RTLGVRPDDD LLFTVFSKGQ KRKMKSLDES ALC IFILKQ INDRIKDRLQ SCYRGEGTLD LAWLKVKDIP CSSALLTIDD NFCGLDMNAP LGVSEMVRGI PVFTEDRDRM TSVI AYVYK NHSLAFVGTK SGKLKKIRVD GPKGNALQYE TVQVVDSGPV LRDMAFSKDH EQLYIMSERQ LTRVPVESCG QYRSC GECL GSGDPHCGWC VLHNTCTRKE RCERSREPRR FASEMKQCVR LTVHPNNISV SQYNVLLVLE TYNVPELSAG VNCTFE DLS EMDGLVIGNQ IQCYSPAAKE VPRIITENGD HHVVQLQLKS KETGMTFAST SFVFYNCSVH NSCLSCVESP YRCHWCK YR HVCTHDPNTC SFQEGRVKLP EDCPQLLRVD KILVPVEVIK PITLKAKNLP QPQSGQRGYE CILNIQGIEQ RVPALRFN S SSVQCQNTSY SYEGMEINNL PVELTVVWNG HFNIDNPAQN KVYLYKCGAM RESCGLCLKA DPDFECGWCQ SPGQCTLRQ HCPAHESRWL ELSGANSKCT NPRITEIIPV TGPREGGTKV TIRGENLGLE FRDIASHVKV AGVECSPLVD GYIPAEQIVC EMGEAKPSQ HAGFVEICVA VCRPEFMARS SQLYYFMTLT LADLKPNRGP MSGGTQVTIT GTNLNAGSNV VVMFGSQPCL F HRRSPSYI ICNTTSSEEV LDMKVTVQVD RARIRQDLVF QYVEDPTIVR IEPEWSIVSG NTPIAVWGTH LDLIQNPQIR AK HGGKEHI NICEVLNATE MTCQAPALAL GPDHQSDLTE RPEEFGFILD NVQSLLILNK TNFTYYPNPV FEAFSPSGIL ELK PGTPII LKGKNLIPPV AGGNVKLNYT VLVGEKPCTV TVSDVQLLCE SPNLIGRHKV MARVGGMEYS PGMVYIAP |
-Macromolecule #4: CALCIUM ION
Macromolecule | Name: CALCIUM ION / type: ligand / ID: 4 / Number of copies: 4 / Formula: CA |
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Molecular weight | Theoretical: 40.078 Da |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7.5 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | C2 aperture diameter: 100.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2.7 mm |
Sample stage | Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN |
Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 50.0 e/Å2 |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |