7LRS

Cryo-EM structure of SARS-CoV-2 spike in complex with neutralizing antibody A23-58.1 that targets the receptor-binding domain

Summary for 7LRS

• Entry DOI: 10.2210/pdb7lrs/pdb
• Related: 7LRT
• EMDB information: 23498 23499 23915
• Descriptor: Spike glycoprotein, antibody A23-58.1 heavy chain, antibody A23-58.1 light chain, ... (5 entities in total)
• Functional Keywords: sars-cov-2, receptor-binding domain, antibody, viral protein-immune system complex, viral protein/immune system
• Biological source: Severe acute respiratory syndrome coronavirus 2 (2019-nCoV, SARS-CoV-2); More
• Total number of polymer chains: 3
• Total formula weight: 71322.45

Authors

Zhou, T.,Tsybovsky, Y. (deposition date: 2021-02-17, release date: 2021-07-14, Last modification date: 2024-11-13)

Primary citation

Wang, L.,Zhou, T.,Zhang, Y.,Yang, E.S.,Schramm, C.A.,Shi, W.,Pegu, A.,Oloniniyi, O.K.,Henry, A.R.,Darko, S.,Narpala, S.R.,Hatcher, C.,Martinez, D.R.,Tsybovsky, Y.,Phung, E.,Abiona, O.M.,Antia, A.,Cale, E.M.,Chang, L.A.,Choe, M.,Corbett, K.S.,Davis, R.L.,DiPiazza, A.T.,Gordon, I.J.,Hait, S.H.,Hermanus, T.,Kgagudi, P.,Laboune, F.,Leung, K.,Liu, T.,Mason, R.D.,Nazzari, A.F.,Novik, L.,O'Connell, S.,O'Dell, S.,Olia, A.S.,Schmidt, S.D.,Stephens, T.,Stringham, C.D.,Talana, C.A.,Teng, I.T.,Wagner, D.A.,Widge, A.T.,Zhang, B.,Roederer, M.,Ledgerwood, J.E.,Ruckwardt, T.J.,Gaudinski, M.R.,Moore, P.L.,Doria-Rose, N.A.,Baric, R.S.,Graham, B.S.,McDermott, A.B.,Douek, D.C.,Kwong, P.D.,Mascola, J.R.,Sullivan, N.J.,Misasi, J.
Ultrapotent antibodies against diverse and highly transmissible SARS-CoV-2 variants.
Science, 373:-, 2021

PubMed Abstract: The emergence of highly transmissible SARS-CoV-2 variants of concern (VOCs) that are resistant to therapeutic antibodies highlights the need for continuing discovery of broadly reactive antibodies. We identified four receptor binding domain-targeting antibodies from three early-outbreak convalescent donors with potent neutralizing activity against 23 variants, including the B.1.1.7, B.1.351, P.1, B.1.429, B.1.526, and B.1.617 VOCs. Two antibodies are ultrapotent, with subnanomolar neutralization titers [half-maximal inhibitory concentration (IC) 0.3 to 11.1 nanograms per milliliter; IC 1.5 to 34.5 nanograms per milliliter). We define the structural and functional determinants of binding for all four VOC-targeting antibodies and show that combinations of two antibodies decrease the in vitro generation of escape mutants, suggesting their potential in mitigating resistance development.

PubMed: 34210892
DOI: 10.1126/science.abh1766

Experimental method

ELECTRON MICROSCOPY (3.89 Å)