7LJY
Cryo-EM structure of the B dENE construct complexed with a 28-mer poly(A)
Summary for 7LJY
| Entry DOI | 10.2210/pdb7ljy/pdb |
| EMDB information | 23401 23402 |
| Descriptor | B dENE construct, poly(A) (2 entities in total) |
| Functional Keywords | rna triple helix, rna stability, poly(a), saxs and cryo-em, rna |
| Biological source | Oryza sativa (rice) More |
| Total number of polymer chains | 2 |
| Total formula weight | 33414.99 |
| Authors | Torabi, S.,Chen, Y.,Zhang, K.,Wang, J.,DeGregorio, S.,Vaidya, A.,Su, Z.,Pabit, S.,Chiu, W.,Pollack, L.,Steitz, J. (deposition date: 2021-02-01, release date: 2021-04-14, Last modification date: 2024-03-06) |
| Primary citation | Torabi, S.F.,Chen, Y.L.,Zhang, K.,Wang, J.,DeGregorio, S.J.,Vaidya, A.T.,Su, Z.,Pabit, S.A.,Chiu, W.,Pollack, L.,Steitz, J.A. Structural analyses of an RNA stability element interacting with poly(A). Proc.Natl.Acad.Sci.USA, 118:-, 2021 Cited by PubMed Abstract: Cis-acting RNA elements are crucial for the regulation of polyadenylated RNA stability. The element for nuclear expression (ENE) contains a U-rich internal loop flanked by short helices. An ENE stabilizes RNA by sequestering the poly(A) tail via formation of a triplex structure that inhibits a rapid deadenylation-dependent decay pathway. Structure-based bioinformatic studies identified numerous ENE-like elements in evolutionarily diverse genomes, including a subclass containing two ENE motifs separated by a short double-helical region (double ENEs [dENEs]). Here, the structure of a dENE derived from a rice transposable element (TWIFB1) before and after poly(A) binding (∼24 kDa and ∼33 kDa, respectively) is investigated. We combine biochemical structure probing, small angle X-ray scattering (SAXS), and cryo-electron microscopy (cryo-EM) to investigate the dENE structure and its local and global structural changes upon poly(A) binding. Our data reveal 1) the directionality of poly(A) binding to the dENE, and 2) that the dENE-poly(A) interaction involves a motif that protects the 3'-most seven adenylates of the poly(A). Furthermore, we demonstrate that the dENE does not undergo a dramatic global conformational change upon poly(A) binding. These findings are consistent with the recently solved crystal structure of a dENE+poly(A) complex [S.-F. Torabi , 371, eabe6523 (2021)]. Identification of additional modes of poly(A)-RNA interaction opens new venues for better understanding of poly(A) tail biology. PubMed: 33785601DOI: 10.1073/pnas.2026656118 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (5.6 Å) |
Structure validation
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