7LGD

HLA-B*07:02 in complex with SARS-CoV-2 nucleocapsid peptide N105-113

Summary for 7LGD

• Entry DOI: 10.2210/pdb7lgd/pdb
• Descriptor: HLA class I histocompatibility antigen, B alpha chain, Beta-2-microglobulin, SARS-CoV-2 nucleocapsid peptide N105-113, ... (7 entities in total)
• Functional Keywords: sars-cov-2, hla, t cell, cross-reactivity, covid-19, antigen presentation, immune system
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 6
• Total formula weight: 91400.72

Authors

Gras, S.,Szeto, C.,Chatzileontiadou, D.S.M. (deposition date: 2021-01-20, release date: 2021-04-21, Last modification date: 2024-10-16)

Primary citation

Lineburg, K.E.,Grant, E.J.,Swaminathan, S.,Chatzileontiadou, D.S.M.,Szeto, C.,Sloane, H.,Panikkar, A.,Raju, J.,Crooks, P.,Rehan, S.,Nguyen, A.T.,Lekieffre, L.,Neller, M.A.,Tong, Z.W.M.,Jayasinghe, D.,Chew, K.Y.,Lobos, C.A.,Halim, H.,Burrows, J.M.,Riboldi-Tunnicliffe, A.,Chen, W.,D'Orsogna, L.,Khanna, R.,Short, K.R.,Smith, C.,Gras, S.
CD8 + T cells specific for an immunodominant SARS-CoV-2 nucleocapsid epitope cross-react with selective seasonal coronaviruses.
Immunity, 54:1055-, 2021

PubMed Abstract: Efforts are being made worldwide to understand the immune response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus responsible for the coronavirus disease 2019 (COVID-19) pandemic, including the impact of T cell immunity and cross-recognition with seasonal coronaviruses. Screening of SARS-CoV-2 peptide pools revealed that the nucleocapsid (N) protein induced an immunodominant response in HLA-B7 COVID-19-recovered individuals that was also detectable in unexposed donors. A single N-encoded epitope that was highly conserved across circulating coronaviruses drove this immunodominant response. In vitro peptide stimulation and crystal structure analyses revealed T cell-mediated cross-reactivity toward circulating OC43 and HKU-1 betacoronaviruses but not 229E or NL63 alphacoronaviruses because of different peptide conformations. T cell receptor (TCR) sequencing indicated that cross-reactivity was driven by private TCR repertoires with a bias for TRBV27 and a long CDR3β loop. Our findings demonstrate the basis of selective T cell cross-reactivity for an immunodominant SARS-CoV-2 epitope and its homologs from seasonal coronaviruses, suggesting long-lasting protective immunity.

PubMed: 33945786
DOI: 10.1016/j.immuni.2021.04.006

Experimental method

X-RAY DIFFRACTION (2.88 Å)