7L9I

Crystal structure of human ARH3-D314A bound to magnesium and ADP-ribose

7L9I の概要

• エントリーDOI: 10.2210/pdb7l9i/pdb
• 分子名称: ADP-ribose glycohydrolase ARH3, [(2R,3S,4R,5R)-5-(6-AMINOPURIN-9-YL)-3,4-DIHYDROXY-OXOLAN-2-YL]METHYL [HYDROXY-[[(2R,3S,4R,5S)-3,4,5-TRIHYDROXYOXOLAN-2-YL]METHOXY]PHOSPHORYL] HYDROGEN PHOSPHATE, MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: adp-ribose, calcium, hydrolase
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 159270.30

構造登録者

Pourfarjam, Y.,Kurinov, I.,Moss, J.,Kim, I.K. (登録日: 2021-01-04, 公開日: 2021-04-28, 最終更新日: 2023-10-18)

主引用文献

Pourfarjam, Y.,Ma, Z.,Kurinov, I.,Moss, J.,Kim, I.K.
Structural and biochemical analysis of human ADP-ribosyl-acceptor hydrolase 3 reveals the basis of metal selectivity and different roles for the two magnesium ions.
J.Biol.Chem., 296:100692-100692, 2021

PubMed Abstract: ADP-ribosylation is a reversible and site-specific post-translational modification that regulates a wide array of cellular signaling pathways. Regulation of ADP-ribosylation is vital for maintaining genomic integrity, and uncontrolled accumulation of poly(ADP-ribosyl)ation triggers a poly(ADP-ribose) (PAR)-dependent release of apoptosis-inducing factor from mitochondria, leading to cell death. ADP-ribosyl-acceptor hydrolase 3 (ARH3) cleaves PAR and mono(ADP-ribosyl)ation at serine following DNA damage. ARH3 is also a metalloenzyme with strong metal selectivity. While coordination of two magnesium ions (Mg and Mg) significantly enhances its catalytic efficiency, calcium binding suppresses its function. However, how the coordination of different metal ions affects its catalysis has not been defined. Here, we report a new crystal structure of ARH3 complexed with its product ADP-ribose and calcium. This structure shows that calcium coordination significantly distorts the binuclear metal center of ARH3, which results in decreased binding affinity to ADP-ribose, and suboptimal substrate alignment, leading to impaired hydrolysis of PAR and mono(ADP-ribosyl)ated serines. Furthermore, combined structural and mutational analysis of the metal-coordinating acidic residues revealed that Mg is crucial for optimal substrate positioning for catalysis, whereas Mg plays a key role in substrate binding. Our collective data provide novel insights into the different roles of these metal ions and the basis of metal selectivity of ARH3 and contribute to understanding the dynamic regulation of cellular ADP-ribosylations during the DNA damage response.

PubMed: 33894202
DOI: 10.1016/j.jbc.2021.100692

実験手法

X-RAY DIFFRACTION (1.8 Å)