7KPD
NMR Solution Structure of Acyclotide ribe 31
Summary for 7KPD
| Entry DOI | 10.2210/pdb7kpd/pdb |
| NMR Information | BMRB: 30813 |
| Descriptor | Acyclotide ribe 31 (1 entity in total) |
| Functional Keywords | antimicrobial protein |
| Biological source | Rinorea bengalensis |
| Total number of polymer chains | 1 |
| Total formula weight | 3139.80 |
| Authors | Harvey, P.J.,Craik, D.J.,Dang, T.T. (deposition date: 2020-11-11, release date: 2021-02-24, Last modification date: 2024-11-20) |
| Primary citation | Dang, T.T.,Chan, L.Y.,Tombling, B.J.,Harvey, P.J.,Gilding, E.K.,Craik, D.J. In Planta Discovery and Chemical Synthesis of Bracelet Cystine Knot Peptides from Rinorea bengalensis . J.Nat.Prod., 2021 Cited by PubMed Abstract: Cyclotides are plant-derived peptides that have attracted interest as biocides and scaffolds for the development of stable peptide therapeutics. Cyclotides are characterized by their cyclic backbone and cystine knot framework, which engenders them with remarkably high stability. This study reports the cystine knot-related peptidome of , a small rainforest tree in the Violaceae family that is distributed from Australia westward to India. Surprisingly, many more acyclic knotted peptides (acyclotides) were discovered than cyclic counterparts (cyclotides), with 32 acyclotides and 1 cyclotide sequenced using combined transcriptome and proteomic analyses. Nine acyclotides were isolated and screened against a panel of mammalian cell lines, showing they had the cytotoxic properties normally associated with cyclotide-like peptides. NMR analysis of the acyclotide ribes 21 and 22 and the cyclotide ribe 33 confirmed that these peptides contained the cystine knot structural motif. The bracelet-subfamily cyclotide ribe 33 was amenable to chemical synthesis in reasonable yield, an achievement that has long eluded previous attempts to synthetically produce bracelet cyclotides. Accordingly, ribe 33 represents an exciting new bracelet cyclotide scaffold that can be subject to chemical modification for future molecular engineering applications. PubMed: 33570395DOI: 10.1021/acs.jnatprod.0c01065 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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