7KOX

Alpha-7 nicotinic acetylcholine receptor bound to epibatidine and PNU-120596 in the activated state

7KOX の概要

• エントリーDOI: 10.2210/pdb7kox/pdb
• EMDBエントリー: 22983
• 分子名称: Neuronal acetylcholine receptor subunit alpha-7,Soluble cytochrome b562 fusion, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (6 entities in total)
• 機能のキーワード: cys-loop receptor, membrane protein
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 324739.38

構造登録者

Noviello, C.M.,Hibbs, R.E.,Gharpure, A.,Mukhtasimova, N.,Baxter, L.,Cabuco, R.,Borek, D.,Sine, S. (登録日: 2020-11-10, 公開日: 2021-03-17, 最終更新日: 2024-10-23)

主引用文献

Noviello, C.M.,Gharpure, A.,Mukhtasimova, N.,Cabuco, R.,Baxter, L.,Borek, D.,Sine, S.M.,Hibbs, R.E.
Structure and gating mechanism of the alpha 7 nicotinic acetylcholine receptor.
Cell, 184:2121-, 2021

PubMed Abstract: The α7 nicotinic acetylcholine receptor plays critical roles in the central nervous system and in the cholinergic inflammatory pathway. This ligand-gated ion channel assembles as a homopentamer, is exceptionally permeable to Ca, and desensitizes faster than any other Cys-loop receptor. The α7 receptor has served as a prototype for the Cys-loop superfamily yet has proven refractory to structural analysis. We present cryo-EM structures of the human α7 nicotinic receptor in a lipidic environment in resting, activated, and desensitized states, illuminating the principal steps in the gating cycle. The structures also reveal elements that contribute to its function, including a C-terminal latch that is permissive for channel opening, and an anionic ring in the extracellular vestibule that contributes to its high conductance and calcium permeability. Comparisons among the α7 structures provide a foundation for mapping the gating cycle and reveal divergence in gating mechanisms in the Cys-loop receptor superfamily.

PubMed: 33735609
DOI: 10.1016/j.cell.2021.02.049

実験手法

ELECTRON MICROSCOPY (2.7 Å)