7KNT
CryoEM structure of the apo-CGRP receptor in a detergent micelle
Summary for 7KNT
| Entry DOI | 10.2210/pdb7knt/pdb |
| EMDB information | 22962 |
| Descriptor | Receptor activity-modifying protein 1, Calcitonin gene-related peptide type 1 receptor (2 entities in total) |
| Functional Keywords | gpcr, cgrp receptor, apo receptor, membrane protein, signaling protein |
| Biological source | Homo sapiens (Human) More |
| Total number of polymer chains | 2 |
| Total formula weight | 73341.22 |
| Authors | Belousoff, M.J.,Danev, R. (deposition date: 2020-11-06, release date: 2021-02-24, Last modification date: 2024-11-06) |
| Primary citation | Josephs, T.M.,Belousoff, M.J.,Liang, Y.L.,Piper, S.J.,Cao, J.,Garama, D.J.,Leach, K.,Gregory, K.J.,Christopoulos, A.,Hay, D.L.,Danev, R.,Wootten, D.,Sexton, P.M. Structure and dynamics of the CGRP receptor in apo and peptide-bound forms. Science, 372:-, 2021 Cited by PubMed Abstract: G protein-coupled receptors (GPCRs) are key regulators of information transmission between cells and organs. Despite this, we have only a limited understanding of the behavior of GPCRs in the apo state and the conformational changes upon agonist binding that lead to G protein recruitment and activation. We expressed and purified unmodified apo and peptide-bound calcitonin gene-related peptide (CGRP) receptors from insect cells to determine their cryo-electron microscopy (cryo-EM) structures, and we complemented these with analysis of protein conformational dynamics using hydrogen-deuterium exchange mass spectrometry and three-dimensional variance analysis of the cryo-EM data. Together with our previously published structure of the active, Gs-bound CGRP receptor complex, our work provides insight into the mechanisms of class B1 GPCR activation. PubMed: 33602864DOI: 10.1126/science.abf7258 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.15 Å) |
Structure validation
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