7KL6
Helicobacter pylori Xanthine-Guanine-Hypoxanthine Phosphoribosyltransferase
Summary for 7KL6
| Entry DOI | 10.2210/pdb7kl6/pdb |
| Descriptor | Phosphoribosyltransferase, (3-{[(4-oxo-4,5-dihydro-3H-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]amino}propyl)phosphonic acid, SULFATE ION, ... (4 entities in total) |
| Functional Keywords | helicobacter pylori xanthine-guanine-hypoxanthine phosphoribosyltransferase drug discovery nucleoside phosphonates, transferase |
| Biological source | Helicobacter pylori |
| Total number of polymer chains | 2 |
| Total formula weight | 37598.78 |
| Authors | Guddat, L.W.,Keough, D.T. (deposition date: 2020-10-29, release date: 2021-05-05, Last modification date: 2024-03-06) |
| Primary citation | Keough, D.T.,Wun, S.J.,Baszczynski, O.,Eng, W.S.,Spacek, P.,Panjikar, S.,Naesens, L.,Pohl, R.,Rejman, D.,Hockova, D.,Ferrero, R.L.,Guddat, L.W. Helicobacter pylori Xanthine-Guanine-Hypoxanthine Phosphoribosyltransferase-A Putative Target for Drug Discovery against Gastrointestinal Tract Infections. J.Med.Chem., 64:5710-5729, 2021 Cited by PubMed Abstract: (Hp) is a human pathogen that lives in the gastric mucosa of approximately 50% of the world's population causing gastritis, peptic ulcers, and gastric cancer. An increase in resistance to current drugs has sparked the search for new Hp drug targets and therapeutics. One target is the disruption of nucleic acid production, which can be achieved by impeding the synthesis of 6-oxopurine nucleoside monophosphates, the precursors of DNA and RNA. These metabolites are synthesized by Hp xanthine-guanine-hypoxanthine phosphoribosyltransferase (XGHPRT). Here, nucleoside phosphonates have been evaluated, which inhibit the activity of this enzyme with values as low as 200 nM. The prodrugs of these compounds arrest the growth of Hp at a concentration of 50 μM in cell-based assays. The kinetic properties of HpXGHPRT have been determined together with its X-ray crystal structure in the absence and presence of 9-[(-3-phosphonopropyl)-aminomethyl-9-deazahypoxanthine, providing a basis for new antibiotic development. PubMed: 33891818DOI: 10.1021/acs.jmedchem.0c02184 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.97 Å) |
Structure validation
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