7KF9

Ebola virus GP (mucin deleted, Makona strain) bound to antibody Fab EBOV-296 and EBOV-515

7KF9 の概要

• エントリーDOI: 10.2210/pdb7kf9/pdb
• EMDBエントリー: 22847
• 分子名称: Antibody Fab EBOV-296 heavy chain, Virion spike glycoprotein 1, Virion spike glycoprotein 2, ... (6 entities in total)
• 機能のキーワード: ebolavirus, antibody, broadly neutralizing, glycan cap, viral protein, viral protein-immune system complex, viral protein/immune system
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 332300.83

構造登録者

Murin, C.D.,Ward, A.B. (登録日: 2020-10-13, 公開日: 2021-04-28, 最終更新日: 2024-10-16)

主引用文献

Murin, C.D.,Gilchuk, P.,Ilinykh, P.A.,Huang, K.,Kuzmina, N.,Shen, X.,Bruhn, J.F.,Bryan, A.L.,Davidson, E.,Doranz, B.J.,Williamson, L.E.,Copps, J.,Alkutkar, T.,Flyak, A.I.,Bukreyev, A.,Crowe Jr., J.E.,Ward, A.B.
Convergence of a common solution for broad ebolavirus neutralization by glycan cap-directed human antibodies.
Cell Rep, 35:108984-108984, 2021

PubMed Abstract: Antibodies that target the glycan cap epitope on the ebolavirus glycoprotein (GP) are common in the adaptive response of survivors. A subset is known to be broadly neutralizing, but the details of their epitopes and basis for neutralization are not well understood. Here, we present cryoelectron microscopy (cryo-EM) structures of diverse glycan cap antibodies that variably synergize with GP base-binding antibodies. These structures describe a conserved site of vulnerability that anchors the mucin-like domains (MLDs) to the glycan cap, which we call the MLD anchor and cradle. Antibodies that bind to the MLD cradle share common features, including use of IGHV1-69 and IGHJ6 germline genes, which exploit hydrophobic residues and form β-hairpin structures to mimic the MLD anchor, disrupt MLD attachment, destabilize GP quaternary structure, and block cleavage events required for receptor binding. Our results provide a molecular basis for ebolavirus neutralization by broadly reactive glycan cap antibodies.

PubMed: 33852862
DOI: 10.1016/j.celrep.2021.108984

実験手法

ELECTRON MICROSCOPY (4.4 Å)