7KDF

Structure of Stu2 Bound to dwarf Ndc80c

Summary for 7KDF

• Entry DOI: 10.2210/pdb7kdf/pdb
• Descriptor: NDC80 isoform 1,NDC80 isoform 1, NUF2 isoform 1,NUF2 isoform 1, Spc24, ... (7 entities in total)
• Functional Keywords: stu2, tension sensing, ndc80, kinetochore, cell cycle
• Biological source: Saccharomyces cerevisiae (Baker's yeast); More
• Total number of polymer chains: 5
• Total formula weight: 87174.69

Authors

Zahm, J.A.,Stewart, M.G.,Miller, M.P.,Harrison, S.C. (deposition date: 2020-10-08, release date: 2020-11-11, Last modification date: 2024-11-06)

Primary citation

Zahm, J.A.,Stewart, M.G.,Carrier, J.S.,Harrison, S.C.,Miller, M.P.
Structural basis of Stu2 recruitment to yeast kinetochores.
Elife, 10:-, 2021

PubMed Abstract: Chromosome segregation during cell division requires engagement of kinetochores of sister chromatids with microtubules emanating from opposite poles. As the corresponding microtubules shorten, these 'bioriented' sister kinetochores experience tension-dependent stabilization of microtubule attachments. The yeast XMAP215 family member and microtubule polymerase, Stu2, associates with kinetochores and contributes to tension-dependent stabilization in vitro. We show here that a C-terminal segment of Stu2 binds the four-way junction of the Ndc80 complex (Ndc80c) and that residues conserved both in yeast Stu2 orthologs and in their metazoan counterparts make specific contacts with Ndc80 and Spc24. Mutations that perturb this interaction prevent association of Stu2 with kinetochores, impair cell viability, produce biorientation defects, and delay cell cycle progression. Ectopic tethering of the mutant Stu2 species to the Ndc80c junction restores wild-type function in vivo. These findings show that the role of Stu2 in tension-sensing depends on its association with kinetochores by binding with Ndc80c.

PubMed: 33591274
DOI: 10.7554/eLife.65389

Experimental method

X-RAY DIFFRACTION (2.72 Å)