7K7X

Solution structure of the cyclotide pase A

Summary for 7K7X

• Entry DOI: 10.2210/pdb7k7x/pdb
• NMR Information: BMRB: 50464
• Descriptor: Cyclotide pase A (1 entity in total)
• Functional Keywords: cyclic peptide, rubiaceae, t-cell proliferation, cytotoxic, hemolytic, immunosuppressant
• Biological source: Psychotria vellosiana
• Total number of polymer chains: 1
• Total formula weight: 2915.37

Authors

Chan, L.Y.,Pinto, M.E.F.,Craik, D.J. (deposition date: 2020-09-24, release date: 2021-08-04, Last modification date: 2024-10-16)

Primary citation

Pinto, M.E.F.,Chan, L.Y.,Koehbach, J.,Devi, S.,Grundemann, C.,Gruber, C.W.,Gomes, M.,Bolzani, V.S.,Cilli, E.M.,Craik, D.J.
Cyclotides from Brazilian Palicourea sessilis and Their Effects on Human Lymphocytes.
J.Nat.Prod., 84:81-90, 2021

PubMed Abstract: Cyclotides are plant-derived peptides found within five families of flowering plants (Violaceae, Rubiaceae, Fabaceae, Solanaceae, and Poaceae) that have a cyclic backbone and six conserved cysteine residues linked by disulfide bonds. Their presence within the Violaceae species seems ubiquitous, yet not all members of other families produce these macrocyclic peptides. The genus Aubl. (Rubiaceae) contains hundreds of neotropical species of shrubs and small trees; however, only a few cyclotides have been discovered hitherto. Herein, five previously uncharacterized Möbius cyclotides within and their pharmacological activities are described. Cyclotides were isolated from leaves and stems of this plant and identified as pase AE, as well as the known peptide kalata S. Cyclotides were de novo sequenced by MALDI-TOF/TOF mass spectrometry, and their structures were solved by NMR spectroscopy. Because some cyclotides have been reported to modulate immune cells, pase AD were assayed for cell proliferation of human primary activated T lymphocytes, and the results showed a dose-dependent antiproliferative function. The toxicity on other nonimmune cells was also assessed. This study reveals that pase cyclotides have potential for applications as immunosuppressants and in immune-related disorders.

PubMed: 33397096
DOI: 10.1021/acs.jnatprod.0c01069

Experimental method

SOLUTION NMR