7K3J

Crystal structure of dLC8 in complex with Panoramix TQT+TQ peptide

7K3J の概要

• エントリーDOI: 10.2210/pdb7k3j/pdb
• 分子名称: Dynein light chain 1, cytoplasmic, Protein panoramix, SULFATE ION (3 entities in total)
• 機能のキーワード: piwi, transposon silencing, heterochromatin formation, pirna pathway, transcriptional silencing, rna binding protein, motor protein
• 由来する生物種: Drosophila melanogaster (Fruit fly); 詳細
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 74794.60

構造登録者

Wang, J.,Patel, D.J. (登録日: 2020-09-11, 公開日: 2021-03-03, 最終更新日: 2023-10-18)

主引用文献

Schnabl, J.,Wang, J.,Hohmann, U.,Gehre, M.,Batki, J.,Andreev, V.I.,Purkhauser, K.,Fasching, N.,Duchek, P.,Novatchkova, M.,Mechtler, K.,Plaschka, C.,Patel, D.J.,Brennecke, J.
Molecular principles of Piwi-mediated cotranscriptional silencing through the dimeric SFiNX complex.
Genes Dev., 35:392-409, 2021

PubMed Abstract: Nuclear Argonaute proteins, guided by their bound small RNAs to nascent target transcripts, mediate cotranscriptional silencing of transposons and repetitive genomic loci through heterochromatin formation. The molecular mechanisms involved in this process are incompletely understood. Here, we show that the SFiNX complex, a silencing mediator downstream from nuclear Piwi-piRNA complexes in , facilitates cotranscriptional silencing as a homodimer. The dynein light chain protein Cut up/LC8 mediates SFiNX dimerization, and its function can be bypassed by a heterologous dimerization domain, arguing for a constitutive SFiNX dimer. Dimeric, but not monomeric SFiNX, is capable of forming molecular condensates in a nucleic acid-stimulated manner. Mutations that prevent SFiNX dimerization result in loss of condensate formation in vitro and the inability of Piwi to initiate heterochromatin formation and silence transposons in vivo. We propose that multivalent SFiNX-nucleic acid interactions are critical for heterochromatin establishment at piRNA target loci in a cotranscriptional manner.

PubMed: 33574069
DOI: 10.1101/gad.347989.120

実験手法

X-RAY DIFFRACTION (2.5 Å)