7K2Y

Crystal structure of CTX-M-14 E166A/K234R Beta-lactamase in complex with hydrolyzed ampicillin

Summary for 7K2Y

Entry DOI	10.2210/pdb7k2y/pdb
Descriptor	Beta-lactamase, (2R,4S)-2-[(1R)-1-{[(2R)-2-amino-2-phenylacetyl]amino}-2-oxoethyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid (3 entities in total)
Functional Keywords	antibiotic resistance, acyl-enzyme intermediate, hydrolase
Biological source	Escherichia coli
Total number of polymer chains	1
Total formula weight	28080.66
Authors	Lu, S.,Palzkill, T.,Sankaran, B.,Hu, L.,Soeung, V.,Prasad, B.V.V. (deposition date: 2020-09-09, release date: 2020-11-04, Last modification date: 2023-10-18)
Primary citation	Soeung, V.,Lu, S.,Hu, L.,Judge, A.,Sankaran, B.,Prasad, B.V.V.,Palzkill, T. A drug-resistant beta-lactamase variant changes the conformation of its active-site proton shuttle to alter substrate specificity and inhibitor potency. J.Biol.Chem., 295:18239-18255, 2020 Cited by PubMed: 33109613 DOI: 10.1074/jbc.RA120.016103 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.62 Å)

Structure validation