7K0T7K0T の概要
| エントリーDOI | 10.2210/pdb7k0t/pdb |
| 関連するPDBエントリー | 7TDG |
| EMDBエントリー | 22596 22597 22615 22616 |
| 分子名称 | RyR1, PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER, ZINC ION (3 entities in total) |
| 機能のキーワード | ryanodine receptor, ryr1, intracellular calcium channel, excitation-contraction coupling, transport protein, atp |
| 由来する生物種 | Oryctolagus cuniculus (rabbit) |
| タンパク質・核酸の鎖数 | 4 |
| 化学式量合計 | 2136935.47 |
| 構造登録者 | |
| 主引用文献 | Nayak, A.R.,Samso, M. Ca 2+ -inactivation of the mammalian ryanodine receptor type 1 in a lipidic environment revealed by cryo-EM. Elife, 11:-, 2022 Cited by PubMed Abstract: Activation of the intracellular Ca channel ryanodine receptor (RyR) triggers a cytosolic Ca surge, while elevated cytosolic Ca inhibits the channel in a negative feedback mechanism. Cryogenic electron microscopy of rabbit RyR1 embedded in nanodiscs under partially inactivating Ca conditions revealed an open and a closed-inactivated conformation. Ca binding to the high-affinity site engages the central and C-terminal domains into a block, which pries the S6 four-helix bundle open. Further rotation of this block pushes S6 toward the central axis, closing (inactivating) the channel. Main characteristics of the Ca-inactivated conformation are downward conformation of the cytoplasmic assembly and tightly knit subunit interface contributed by a fully occupied Ca activation site, two inter-subunit resolved lipids, and two salt bridges between the EF hand domain and the S2-S3 loop validated by disease-causing mutations. The structural insight illustrates the prior Ca activation prerequisite for Ca inactivation and provides for a seamless transition from inactivated to closed conformations. PubMed: 35257661DOI: 10.7554/eLife.75568 主引用文献が同じPDBエントリー |
| 実験手法 | ELECTRON MICROSCOPY (4.3 Å) |
構造検証レポート
検証レポート(詳細版)
をダウンロード
をダウンロード
