7JZH

The Cryo-EM structure of the Glutamate decarboxylase from Escherichia coli

Summary for 7JZH

• Entry DOI: 10.2210/pdb7jzh/pdb
• EMDB information: 22531
• Descriptor: Glutamate decarboxylase (1 entity in total)
• Functional Keywords: glutamate decarboxylase beta, oxidoreductase, lyase
• Biological source: Escherichia coli
• Total number of polymer chains: 6
• Total formula weight: 316367.74

Authors

Su, C.-C. (deposition date: 2020-09-02, release date: 2021-01-20, Last modification date: 2024-03-06)

Primary citation

Su, C.C.,Lyu, M.,Morgan, C.E.,Bolla, J.R.,Robinson, C.V.,Yu, E.W.
A 'Build and Retrieve' methodology to simultaneously solve cryo-EM structures of membrane proteins.
Nat.Methods, 18:69-75, 2021

PubMed Abstract: Single-particle cryo-electron microscopy (cryo-EM) has become a powerful technique in the field of structural biology. However, the inability to reliably produce pure, homogeneous membrane protein samples hampers the progress of their structural determination. Here, we develop a bottom-up iterative method, Build and Retrieve (BaR), that enables the identification and determination of cryo-EM structures of a variety of inner and outer membrane proteins, including membrane protein complexes of different sizes and dimensions, from a heterogeneous, impure protein sample. We also use the BaR methodology to elucidate structural information from Escherichia coli K12 crude membrane and raw lysate. The findings demonstrate that it is possible to solve high-resolution structures of a number of relatively small (<100 kDa) and less abundant (<10%) unidentified membrane proteins within a single, heterogeneous sample. Importantly, these results highlight the potential of cryo-EM for systems structural proteomics.

PubMed: 33408407
DOI: 10.1038/s41592-020-01021-2

Experimental method

ELECTRON MICROSCOPY (3.59 Å)