7JSV

Cryo-EM structure of conjugative pili from carbapenem-resistant Klebsiella pneumoniae

Summary for 7JSV

• Entry DOI: 10.2210/pdb7jsv/pdb
• EMDB information: 22460
• Descriptor: Pilin, 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE (2 entities in total)
• Functional Keywords: conjugation pili, helical reconstruction, protein fibril
• Biological source: Klebsiella pneumoniae
• Total number of polymer chains: 41
• Total formula weight: 329952.95

Authors

Zheng, W.,Pena, A.,Frankel, G.,Egelman, E.H. (deposition date: 2020-08-16, release date: 2020-09-02, Last modification date: 2024-03-06)

Primary citation

Zheng, W.,Pena, A.,Low, W.W.,Wong, J.L.C.,Frankel, G.,Egelman, E.H.
Cryoelectron-Microscopic Structure of the pKpQIL Conjugative Pili from Carbapenem-Resistant Klebsiella pneumoniae.
Structure, 28:1321-, 2020

PubMed Abstract: Conjugative pili are important in mediating bacterial conjugation and horizontal gene transfer. Since plasmid transfer can include antibiotic-resistance genes, conjugation is an important mechanism in the spread of antibiotic resistance. Filamentous bacteriophages have been shown to exist in two different structural classes: those with a 5-fold rotational symmetry and those with a one-start helix with approximately 5 subunits per turn. Structures for the F and the F-like pED208 conjugation pilus have shown that they have 5-fold rotational symmetry. Here, we report the cryoelectron-microscopic structure of conjugative pili from carbapenem-resistant Klebsiella pneumoniae, encoded on the IncFIIK pKpQIL plasmid, at 3.9 Å resolution and show that it has a one-start helix. These results establish that conjugation pili can exist in at least two structural classes, consistent with other results showing that relatively small perturbations are needed to change the helical symmetry of polymers.

PubMed: 32916103
DOI: 10.1016/j.str.2020.08.010

Experimental method

ELECTRON MICROSCOPY (3.9 Å)