7JSA

Crystal structure of the DNA binding domain of human transcription factor ERF in the reduced form, in complex with double-stranded DNA ACCGGAAGTG

7JSA の概要

• エントリーDOI: 10.2210/pdb7jsa/pdb
• 分子名称: DNA (5'-D(*AP*CP*CP*GP*GP*AP*AP*GP*TP*G)-3'), DNA (5'-D(*CP*AP*CP*TP*TP*CP*CP*GP*GP*T)-3'), ETS domain-containing transcription factor ERF, ... (4 entities in total)
• 機能のキーワード: transcription, tumor suppressor, ets family, repressor, dna binding protein, dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 20312.47

構造登録者

Hou, C.,Tsodikov, O.V. (登録日: 2020-08-14, 公開日: 2020-11-25, 最終更新日: 2023-10-18)

主引用文献

Hou, C.,McCown, C.,Ivanov, D.N.,Tsodikov, O.V.
Structural Insight into the DNA Binding Function of Transcription Factor ERF.
Biochemistry, 2020

PubMed Abstract: ETS family transcription factors control development of different cell types in humans, whereas deregulation of these proteins leads to severe developmental syndromes and cancers. One of a few members of the ETS family that are known to act solely as repressors, ERF, is required for normal osteogenesis and hematopoiesis. Another important function of ERF is acting as a tumor suppressor by antagonizing oncogenic fusions involving other ETS family factors. The structure of ERF and the DNA binding properties specific to this protein have not been elucidated. In this study, we determined two crystal structures of the complexes of the DNA binding domain of ERF with DNA. In one, ERF is in a distinct dimeric form, with Cys72 in a reduced state. In the other, two dimers of ERF are assembled into a tetramer that is additionally locked by two Cys72-Cys72 disulfide bonds across the dimers. In the tetramer, the ERF molecules are bound to a pseudocontinuous DNA on the same DNA face at two GGAA binding sites on opposite strands. Sedimentation velocity analysis showed that this tetrameric assembly forms on continuous DNA containing such tandem sites spaced by 7 bp. Our bioinformatic analysis of three previously reported sets of ERF binding loci across entire genomes showed that these loci were enriched in such 7 bp spaced tandem sites. Taken together, these results strongly suggest that the observed tetrameric assembly is a functional state of ERF in the human cell.

PubMed: 33175491
DOI: 10.1021/acs.biochem.0c00774

実験手法

X-RAY DIFFRACTION (2.85 Å)