7JSA

Crystal structure of the DNA binding domain of human transcription factor ERF in the reduced form, in complex with double-stranded DNA ACCGGAAGTG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 41 21 2
Unit cell lengths	63.798, 63.798, 137.502
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.750 - 2.850
R-factor	0.2483
Rwork	0.247
R-free	0.27530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6jvt
RMSD bond length	0.004
RMSD bond angle	1.149
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.850	2.850
Rmerge	0.090	0.440
Number of reflections	7139	348
<I/σ(I)>	32	2.06
Completeness [%]	99.9
Redundancy	10.8
CC(1/2)		0.890

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	294	6% PEG 4000, 0.1M Hepes, 7.8