7JRG
Plant Mitochondrial complex III2 from Vigna radiata
Summary for 7JRG
| Entry DOI | 10.2210/pdb7jrg/pdb |
| EMDB information | 22445 |
| Descriptor | Mitochondrial-processing peptidase subunit beta, mitochondrial isoform X1, QCR10, ZINC ION, ... (16 entities in total) |
| Functional Keywords | mitochondria, respiration, bioenergetics, plants, electron transport |
| Biological source | Vigna radiata var. radiata (Mung bean) More |
| Total number of polymer chains | 20 |
| Total formula weight | 570542.36 |
| Authors | Maldonado, M.,Letts, J.A. (deposition date: 2020-08-12, release date: 2021-01-20, Last modification date: 2024-11-20) |
| Primary citation | Maldonado, M.,Guo, F.,Letts, J.A. Atomic structures of respiratory complex III 2 , complex IV, and supercomplex III 2 -IV from vascular plants. Elife, 10:-, 2021 Cited by PubMed Abstract: Mitochondrial complex III (CIII) and complex IV (CIV), which can associate into a higher-order supercomplex (SC III+IV), play key roles in respiration. However, structures of these plant complexes remain unknown. We present atomic models of CIII, CIV, and SC III+IV from determined by single-particle cryoEM. The structures reveal plant-specific differences in the MPP domain of CIII and define the subunit composition of CIV. Conformational heterogeneity analysis of CIII revealed long-range, coordinated movements across the complex, as well as the motion of CIII's iron-sulfur head domain. The CIV structure suggests that, in plants, proton translocation does not occur via the H channel. The supercomplex interface differs significantly from that in yeast and bacteria in its interacting subunits, angle of approach and limited interactions in the mitochondrial matrix. These structures challenge long-standing assumptions about the plant complexes and generate new mechanistic hypotheses. PubMed: 33463523DOI: 10.7554/eLife.62047 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.2 Å) |
Structure validation
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