7JJK

Crystal structure of SOX30

7JJK の概要

• エントリーDOI: 10.2210/pdb7jjk/pdb
• 分子名称: Transcription factor SOX-30 (2 entities in total)
• 機能のキーワード: high mobility group, sox30, sox, transcription factor, hmg box, transcription
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 10502.07

構造登録者

Ghafoori, S.M.,Forwood, J.K. (登録日: 2020-07-27, 公開日: 2020-08-19, 最終更新日: 2025-09-10)

主引用文献

Ghafoori, S.M.,Sethi, A.,Petersen, G.F.,Tanipour, M.H.,Gooley, P.R.,Forwood, J.K.
RNA Binding Properties of SOX Family Members.
Cells, 13:-, 2024

PubMed Abstract: SOX proteins are a family of transcription factors (TFs) that play critical functions in sex determination, neurogenesis, and chondrocyte differentiation, as well as cardiac, vascular, and lymphatic development. There are 20 SOX family members in humans, each sharing a 79-residue L-shaped high mobility group (HMG)-box domain that is responsible for DNA binding. SOX2 was recently shown to interact with long non-coding RNA and large-intergenic non-coding RNA to regulate embryonic stem cell and neuronal differentiation. The RNA binding region was shown to reside within the HMG-box domain; however, the structural details of this binding remain unclear. Here, we show that all SOX family members, except group H, interact with RNA. Our mutational experiments demonstrate that the disordered C-terminal region of the HMG-box domain plays an important role in RNA binding. Further, by determining a high-resolution structure of the HMG-box domain of the group H family member SOX30, we show that despite differences in RNA binding ability, SOX30 shares a very similar secondary structure with other SOX protein HMG-box domains. Together, our study provides insight into the interaction of SOX TFs with RNA.

PubMed: 39056784
DOI: 10.3390/cells13141202

実験手法

X-RAY DIFFRACTION (1.4 Å)