7FGH

Cryo-EM Structure of Chikungunya Virus Nonstructural Protein 1 with m7GMP

Summary for 7FGH

• Entry DOI: 10.2210/pdb7fgh/pdb
• EMDB information: 31581
• Descriptor: mRNA-capping enzyme nsP1, ZINC ION, S-ADENOSYL-L-HOMOCYSTEINE, ... (5 entities in total)
• Functional Keywords: nonstructural protein, chikungunya virus, rna cap, replication, viral protein
• Biological source: Chikungunya virus strain S27-African prototype (CHIKV)
• Total number of polymer chains: 12
• Total formula weight: 754615.37

Authors

Zhang, K.,Law, M.C.Y.,Nguyen, T.M.,Tan, Y.B.,Wirawan, M.,Law, Y.S.,Luo, D.H. (deposition date: 2021-07-27, release date: 2022-07-27, Last modification date: 2022-08-10)

Primary citation

Zhang, K.,Law, M.C.Y.,Nguyen, T.M.,Tan, Y.B.,Wirawan, M.,Law, Y.S.,Jeong, L.S.,Luo, D.
Molecular basis of specific viral RNA recognition and 5'-end capping by the Chikungunya virus nsP1.
Cell Rep, 40:111133-111133, 2022

PubMed Abstract: Many viruses encode RNA-modifying enzymes to edit the 5' end of viral RNA to mimic the cellular mRNA for effective protein translation, genome replication, and evasion of the host defense mechanisms. Alphavirus nsP1 synthesizes the 5' end Cap-0 structure of viral RNAs. However, the molecular basis of the capping process remains unclear. We determine high-resolution cryoelectron microscopy (cryo-EM) structures of Chikungunya virus nsP1 in complex with m7GTP/SAH, covalently attached m7GMP, and Cap-0 viral RNA. These structures reveal details of viral-RNA-capping reactions and uncover a sequence-specific virus RNA-recognition pattern that, in turn, regulates viral-RNA-capping efficiency to ensure optimal genome replication and subgenomic RNA transcription. This sequence-specific enzyme-RNA pairing is conserved across all alphaviruses.

PubMed: 35905713
DOI: 10.1016/j.celrep.2022.111133

Experimental method

ELECTRON MICROSCOPY (2.18 Å)