7FDF
The E145S mutant of the regulatory domain of YeiE, a sulfite sensing LysR-type transcriptional regulator from Cronobacter sakazakii (sulfate-bound form)
Summary for 7FDF
| Entry DOI | 10.2210/pdb7fdf/pdb |
| Related | 7ERP 7ERQ |
| Descriptor | LysR family transcriptional regulator, SULFATE ION (3 entities in total) |
| Functional Keywords | lysr-type transcriptional regulator, lttr, cronobacter sakazakii, sulfite, dna binding protein |
| Biological source | Cronobacter sakazakii (Enterobacter sakazakii) |
| Total number of polymer chains | 1 |
| Total formula weight | 23318.00 |
| Authors | |
| Primary citation | Hong, S.,Kim, J.,Cho, E.,Na, S.,Yoo, Y.J.,Cho, Y.H.,Ryu, S.,Ha, N.C. Crystal structures of YeiE from Cronobacter sakazakii and the role of sulfite tolerance in gram-negative bacteria. Proc.Natl.Acad.Sci.USA, 119:e2118002119-e2118002119, 2022 Cited by PubMed Abstract: SignificanceYeiE has been identified as a master virulence factor of . In this study, we determined the crystal structures of the regulatory domain of YeiE in complex with its physiological ligand sulfite ion (SO). The structure provides the basis for the molecular mechanisms for sulfite sensing and the ligand-dependent conformational changes of the regulatory domain. The genes under the control of YeiE in response to sulfite were investigated to reveal the functional roles of YeiE in the sulfite tolerance of the bacteria. We propose the molecular mechanism underlying the ability of gram-negative pathogens to defend against the innate immune response involving sulfite, thus providing a strategy to control the pathogenesis of bacteria. PubMed: 35271389DOI: 10.1073/pnas.2118002119 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.05 Å) |
Structure validation
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