7FAB

CRYSTAL STRUCTURE OF HUMAN IMMUNOGLOBULIN FRAGMENT FAB NEW REFINED AT 2.0 ANGSTROMS RESOLUTION

「3FAB」から置き換えられました 「1FAB」から置き換えられました 「2FAB」から置き換えられました

7FAB の概要

• エントリーDOI: 10.2210/pdb7fab/pdb
• 分子名称: IGG1-LAMBDA NEW FAB (LIGHT CHAIN), IGG1-LAMBDA NEW FAB (HEAVY CHAIN) (3 entities in total)
• 機能のキーワード: immunoglobulin, immune system
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 45060.44

構造登録者

Saul, F.,Poljak, R.J. (登録日: 1991-11-20, 公開日: 1994-01-31, 最終更新日: 2024-10-16)

主引用文献

Saul, F.A.,Poljak, R.J.
Crystal structure of human immunoglobulin fragment Fab New refined at 2.0 A resolution.
Proteins, 14:363-371, 1992

PubMed Abstract: The three-dimensional structure of the human immunoglobulin fragment Fab New (IgG1, lambda) has been refined to a crystallographic R-factor of 16.9% to 2 A resolution. Rms deviations of the final model from ideal geometry are 0.014 A for bond distances and 3.03 degrees for bond angles. Refinement was based on a new X-ray data set including 28,301 reflections with F > 2.5 sigma(F) from 6.0 to 2.0 A resolution. The starting model for the refinement procedure reported here is from the Brookhaven Protein Data Bank entry 3FAB (rev. 1981). Differences between the initial and final models include modified polypeptide-chain folding in the third complementarity-determining region (CDR3) and the third framework region (FR3) of VH and in some exposed loops of CL and CH1. Amino acid sequence changes were determined at a number of positions by inspection of difference electron density maps. The incorporation of amino acid sequence changes results in an improved VH framework model for the "humanization" of monoclonal antibodies.

PubMed: 1438175
DOI: 10.1002/prot.340140305

実験手法

X-RAY DIFFRACTION (2 Å)