7F9M

Crystal structure of the variable region of Plasmodium RIFIN #4 (PF3D7_1000500) in complex with LAIR1 (with T67L, N69S and A77T mutations)

7F9M の概要

• エントリーDOI: 10.2210/pdb7f9m/pdb
• 分子名称: Rifin, Leukocyte-associated immunoglobulin-like receptor 1 (2 entities in total)
• 機能のキーワード: malaria, plasmodium falciparum, rifin, lair1, immune system
• 由来する生物種: Plasmodium falciparum (isolate 3D7); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 62704.78

構造登録者

Xie, Y.,Song, H.,Li, X.,Qi, J.,Gao, G.F. (登録日: 2021-07-04, 公開日: 2021-08-18, 最終更新日: 2024-10-16)

主引用文献

Xie, Y.,Li, X.,Chai, Y.,Song, H.,Qi, J.,Gao, G.F.
Structural basis of malarial parasite RIFIN-mediated immune escape against LAIR1.
Cell Rep, 36:109600-109600, 2021

PubMed Abstract: Malaria infection by Plasmodium falciparum continues to pose a global threat to the human population. P. falciparum expresses variable erythrocyte surface antigens such as RIFINs. Public antibodies with LAIR1 insertion have been identified from malarial patients against a subset of RIFINs. In this study, we solve a LAIR1-binding RIFIN structure: the complex structures of two RIFINs bound to mutated or wild-type LAIR1 in two distinct patterns. Notably, the two RIFINs engage similar binding sites on LAIR1 with different angles, and the RIFIN-binding sites overlap with the collagen-binding site. Surprisingly, RIFINs use completely different binding sites to bind to LAIR1 or LILRB1, indicating the kaleidoscopic change of RIFINs. We then verify that RIFIN could induce LAIR1-mediated cell signaling, and LAIR1-containing antibodies could block the pathway. The findings of this study provide structural insights into the mechanism of the immune escape of P. falciparum and the endless arms race between parasite and host.

PubMed: 34433057
DOI: 10.1016/j.celrep.2021.109600

実験手法

X-RAY DIFFRACTION (2.9 Å)