7F79

Crystal structure of glutamate dehydrogenase 3 from Candida albicans in complex with alpha-ketoglutarate and NADPH

7F79 の概要

• エントリーDOI: 10.2210/pdb7f79/pdb
• 関連するPDBエントリー: 7F77
• 分子名称: Glutamate dehydrogenase, NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE, 2-OXOGLUTARIC ACID, ... (5 entities in total)
• 機能のキーワード: candida albicans, yeast-to-hyphal transition, glutamate dehydrogenase, conformational change, oxidoreductase
• 由来する生物種: Candida albicans SC5314 (Yeast)
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 323044.63

構造登録者

Li, N.,Wang, W.,Zeng, X.,Liu, M.,Li, M.,Li, C.,Wang, M. (登録日: 2021-06-28, 公開日: 2021-07-21, 最終更新日: 2023-11-29)

主引用文献

Li, N.,Wang, W.,Zeng, X.,Liu, M.,Li, M.,Li, C.,Wang, M.
Crystal structure of glutamate dehydrogenase 3 from Candida albicans.
Biochem.Biophys.Res.Commun., 570:15-20, 2021

PubMed Abstract: Glutamate dehydrogenase 3 from Candida albicans (CaGdh3) catalyzes the reversible oxidative deamination of l-glutamate, playing an important role in the yeast-to-hyphal transition of C. albicans. Here we report the crystal structures of CaGdh3 and its complex with α-ketoglutarate and NADPH. CaGdh3 exists as a hexamer, with each subunit containing two domains. The substrate and coenzyme bind in the cleft between the two domains and their binding induces a conformational change in CaGdh3. Our results will help to understand the catalytic mechanism of CaGdh3 and will provide a structural basis for the design of antifungal drugs targeting the CaGdh3 pathway.

PubMed: 34271431
DOI: 10.1016/j.bbrc.2021.07.014

実験手法

X-RAY DIFFRACTION (2.7 Å)