7F6L

Crystal structure of human MUS81-EME2 complex

7F6L の概要

• エントリーDOI: 10.2210/pdb7f6l/pdb
• 分子名称: Crossover junction endonuclease MUS81, Probable crossover junction endonuclease EME2 (2 entities in total)
• 機能のキーワード: mus81, eme2, endonuclease, dna binding protein
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 75240.54

構造登録者

Hua, Z.K.,Zhang, D.P.,Yuan, C.,Lin, Z.H. (登録日: 2021-06-25, 公開日: 2022-03-02, 最終更新日: 2023-11-29)

主引用文献

Hua, Z.,Fang, Q.,Zhang, D.,Luo, Z.,Yuan, C.,Lin, Z.
Crystal structure of the human MUS81-EME2 complex.
Structure, 30:743-752.e3, 2022

PubMed Abstract: MUS81 is an important structure-specific endonuclease responsible for the processing of stalled replication forks and recombination intermediates. In human, MUS81 functions by forming complexes with its regulatory subunits EME1 and EME2, playing distinct roles in G2/M and S phases. Although the structures of MUS81-EME1 have been intensively studied, there is no structure information available about MUS81-EME2. Here, we report the crystal structure of MUS81-EME2, which reveals an overall protein fold similar to that of MUS81-EME1 complex. Further biochemical and structural characterization shows that the MUS81-EME1 and MUS81-EME2 complexes are identical in substrate recognition and endonuclease activities in vitro, implying that the distinct cellular roles of the two complexes could arise from temporal controls in cells. Finally, an extensive structure-guided mutagenesis analysis provides implications for the molecular basis of how the MUS81-EME endonucleases recognize various DNA substrates in a structure-selective manner.

PubMed: 35290797
DOI: 10.1016/j.str.2022.02.015

実験手法

X-RAY DIFFRACTION (3.2 Å)