7F5J
The crystal structure of VyPAL2-I244V, a more efficient mutant of VyPAL2 peptide asparaginyl ligase in its active enzyme form
7F5J の概要
エントリーDOI | 10.2210/pdb7f5j/pdb |
関連するPDBエントリー | 6IDV |
分子名称 | Peptide Asparaginyl Ligases, alpha-L-fucopyranose-(1-6)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (7 entities in total) |
機能のキーワード | aep, legumain, peptide ligase, pal, plant protein |
由来する生物種 | Viola philippica |
タンパク質・核酸の鎖数 | 2 |
化学式量合計 | 64655.00 |
構造登録者 | |
主引用文献 | Hu, S.,El Sahili, A.,Kishore, S.,Wong, Y.H.,Hemu, X.,Goh, B.C.,Zhipei, S.,Wang, Z.,Tam, J.P.,Liu, C.F.,Lescar, J. Structural basis for proenzyme maturation, substrate recognition, and ligation by a hyperactive peptide asparaginyl ligase. Plant Cell, 34:4936-4949, 2022 Cited by PubMed Abstract: Peptide ligases are versatile enzymes that can be utilized for precise protein conjugation for bioengineering applications. Hyperactive peptide asparaginyl ligases (PALs), such as butelase-1, belong to a small class of enzymes from cyclotide-producing plants that can perform site-specific, rapid ligation reactions after a target peptide asparagine/aspartic acid (Asx) residue binds to the active site of the ligase. How PALs specifically recognize their polypeptide substrates has remained elusive, especially at the prime binding side of the enzyme. Here we report crystal structures that capture VyPAL2, a catalytically efficient PAL from Viola yedoensis, in an activated state, with and without a bound substrate. The bound structure shows one ligase with the N-terminal polypeptide tail from another ligase molecule trapped at its active site, revealing how Asx inserts in the enzyme's S1 pocket and why a hydrophobic residue is required at the P2' position. Besides illustrating the anchoring role played by P1 and P2' residues, these results uncover a role for the Gatekeeper residue at the surface of the S2 pocket in shifting the nonprime portion of the substrate and, as a result, the activity toward ligation or hydrolysis. These results suggest a picture for proenzyme maturation in the vacuole and will inform the rational design of peptide ligases with tailored specificities. PubMed: 36099055DOI: 10.1093/plcell/koac281 主引用文献が同じPDBエントリー |
実験手法 | X-RAY DIFFRACTION (1.593 Å) |
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