7F4Y

Crystal structure of replisomal dimer of DNA polymerase from bacteriophage RB69 with DNA duplexes

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7F4Y の概要

• エントリーDOI: 10.2210/pdb7f4y/pdb
• 分子名称: DNA polymerase, DNA (5'-D(*TP*CP*AP*AP*GP*TP*AP*AP*GP*CP*AP*GP*TP*CP*CP*GP*CP*TP*C)-3'), DNA (5'-D(*GP*AP*GP*CP*GP*GP*AP*CP*TP*GP*CP*TP*TP*AP*C)-3'), ... (8 entities in total)
• 機能のキーワード: transferase-dna complex, transferase/dna
• 由来する生物種: Enterobacteria phage RB69; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 232214.79

構造登録者

Youn, H.-S.,Park, J.,An, J.Y.,Lee, Y.,Eom, S.H.,Wang, J. (登録日: 2021-06-21, 公開日: 2021-07-14, 最終更新日: 2023-11-29)

主引用文献

Park, J.,Youn, H.S.,An, J.Y.,Lee, Y.,Eom, S.H.,Wang, J.
Structure of New Binary and Ternary DNA Polymerase Complexes From Bacteriophage RB69.
Front Mol Biosci, 8:704813-704813, 2021

PubMed Abstract: DNA polymerase plays a critical role in passing the genetic information of any living organism to its offspring. DNA polymerase from enterobacteria phage RB69 (RB69pol) has both polymerization and exonuclease activities and has been extensively studied as a model system for B-family DNA polymerases. Many binary and ternary complex structures of RB69pol are known, and they all contain a single polymerase-primer/template (P/T) DNA complex. Here, we report a crystal structure of the exonuclease-deficient RB69pol with the P/T duplex in a dimeric form at a resolution of 2.2 Å. The structure includes one new closed ternary complex with a single divalent metal ion bound and one new open binary complex in the pre-insertion state with a vacant dNTP-binding pocket. These complexes suggest that initial binding of the correct dNTP in the open state is much weaker than expected and that initial binding of the second divalent metal ion in the closed state is also much weaker than measured. Additional conformational changes are required to convert these complexes to high-affinity states. Thus, the measured affinities for the correct incoming dNTP and divalent metal ions are average values from many conformationally distinctive states. Our structure provides new insights into the order of the complex assembly involving two divalent metal ions. The biological relevance of specific interactions observed between one RB69pol and the P/T duplex bound to the second RB69pol observed within this dimeric complex is discussed.

PubMed: 34869578
DOI: 10.3389/fmolb.2021.704813

実験手法

X-RAY DIFFRACTION (2.2 Å)