7F1K
Designed enzyme RA61 M48K/I72D mutant: form IV
Summary for 7F1K
| Entry DOI | 10.2210/pdb7f1k/pdb |
| Descriptor | Engineered Retroaldolase (2 entities in total) |
| Functional Keywords | aldol reaction, retro-aldol reaction, mutation, reaction direction, de novo protein |
| Biological source | synthetic construct |
| Total number of polymer chains | 1 |
| Total formula weight | 22187.08 |
| Authors | Fujioka, T.,Oka, M.,Numoto, N.,Ito, N.,Oda, M.,Tanaka, F. (deposition date: 2021-06-09, release date: 2021-11-24, Last modification date: 2023-11-29) |
| Primary citation | Fujioka, T.,Numoto, N.,Akama, H.,Shilpa, K.,Oka, M.,Roy, P.K.,Krishna, Y.,Ito, N.,Baker, D.,Oda, M.,Tanaka, F. Varying the Directionality of Protein Catalysts for Aldol and Retro-Aldol Reactions. Chembiochem, 23:e202100435-e202100435, 2022 Cited by PubMed Abstract: Natural aldolase enzymes and created retro-aldolase protein catalysts often catalyze both aldol and retro-aldol reactions depending on the concentrations of the reactants and the products. Here, we report that the directionality of protein catalysts can be altered by replacing one amino acid. The protein catalyst derived from a scaffold of a previously reported retro-aldolase catalyst, catalyzed aldol reactions more efficiently than the previously reported retro-aldolase catalyst. The retro-aldolase catalyst efficiently catalyzed the retro-aldol reaction but was less efficient in catalyzing the aldol reaction. The results indicate that protein catalysts with varying levels of directionality in usually reversibly catalyzed aldol and retro-aldol reactions can be generated from the same protein scaffold. PubMed: 34698422DOI: 10.1002/cbic.202100435 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.05 Å) |
Structure validation
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