7EUC

Parallel G-quadruplex structure

Summary for 7EUC

• Entry DOI: 10.2210/pdb7euc/pdb
• Descriptor: DNA (5'-D(*TP*TP*GP*GP*GP*G)-3'), DNA (5'-D(P*GP*GP*GP*G)-3'), DNA (5'-D(P*GP*GP*GP*GP*T)-3'), ... (4 entities in total)
• Functional Keywords: g-quadruplex, four-layer, parallel, l-serinol, stable, dna
• Biological source: synthetic construct; More
• Total number of polymer chains: 4
• Total formula weight: 7026.58

Authors

Winnerdy, F.R.,Devi, G.,Ang, J.C.Y.,Lim, K.W.,Phan, A.T. (deposition date: 2021-05-17, release date: 2022-03-30, Last modification date: 2024-05-15)

Primary citation

Devi, G.,Winnerdy, F.R.,Ang, J.C.Y.,Lim, K.W.,Phan, A.T.
Four-Layered Intramolecular Parallel G-Quadruplex with Non-Nucleotide Loops: An Ultra-Stable Self-Folded DNA Nano-Scaffold.
Acs Nano, 16:533-540, 2022

PubMed Abstract: A four-stranded scaffold of nucleic acids termed G-quadruplex (G4) has found growing applications in nano- and biotechnology. Propeller loops are a hallmark of the most stable intramolecular parallel-stranded G4s. To date, propeller loops have been observed to span only a maximum of three G-tetrad layers. Going beyond that would allow creation of more stable scaffolds useful for building robust nanodevices. Here we investigate the formation of propeller loops spanning more than three layers. We show that native nucleotide sequences are incompatible toward this goal, and we report on synthetic non-nucleotide linkers that form a propeller loop across four layers. With the established linkers, we constructed a four-layered intramolecular parallel-stranded G4, which exhibited ultrahigh thermal stability. Control on loop design would augment the toolbox toward engineering of G4-based nanoscaffolds for diverse applications.

PubMed: 34927423
DOI: 10.1021/acsnano.1c07630

Experimental method

SOLUTION NMR