7ERQ

The regulatory domain of YeiE, a sulfite sensing LysR-type transcriptional regulator from Cronobacter sakazakii (ligand-free form)

Summary for 7ERQ

• Entry DOI: 10.2210/pdb7erq/pdb
• Related: 7ERP
• Descriptor: LysR family transcriptional regulator (2 entities in total)
• Functional Keywords: lysr-type transcriptional regulator, lttr, cronobacter sakazakii, sulfite, dna binding protein
• Biological source: Cronobacter sakazakii (Enterobacter sakazakii)
• Total number of polymer chains: 2
• Total formula weight: 46559.94

Authors

Hong, S.,Ha, N.-C. (deposition date: 2021-05-06, release date: 2022-03-23, Last modification date: 2024-10-09)

Primary citation

Hong, S.,Kim, J.,Cho, E.,Na, S.,Yoo, Y.J.,Cho, Y.H.,Ryu, S.,Ha, N.C.
Crystal structures of YeiE from Cronobacter sakazakii and the role of sulfite tolerance in gram-negative bacteria.
Proc.Natl.Acad.Sci.USA, 119:e2118002119-e2118002119, 2022

PubMed Abstract: SignificanceYeiE has been identified as a master virulence factor of . In this study, we determined the crystal structures of the regulatory domain of YeiE in complex with its physiological ligand sulfite ion (SO). The structure provides the basis for the molecular mechanisms for sulfite sensing and the ligand-dependent conformational changes of the regulatory domain. The genes under the control of YeiE in response to sulfite were investigated to reveal the functional roles of YeiE in the sulfite tolerance of the bacteria. We propose the molecular mechanism underlying the ability of gram-negative pathogens to defend against the innate immune response involving sulfite, thus providing a strategy to control the pathogenesis of bacteria.

PubMed: 35271389
DOI: 10.1073/pnas.2118002119

Experimental method

X-RAY DIFFRACTION (1.95 Å)