7EQ9
Cryo-EM structure of designed protein nanoparticle TIP60 (Truncated Icosahedral Protein composed of 60-mer fusion proteins)
This is a non-PDB format compatible entry.
Summary for 7EQ9
| Entry DOI | 10.2210/pdb7eq9/pdb |
| EMDB information | 31256 |
| Descriptor | TIP60 (1 entity in total) |
| Functional Keywords | artificial designed protein complex, virus like particle, de novo protein |
| Biological source | synthetic construct |
| Total number of polymer chains | 60 |
| Total formula weight | 1067653.62 |
| Authors | Obata, J.,Kawakami, N.,Tsutsumi, A.,Miyamoto, K.,Kikkawa, M.,Arai, R. (deposition date: 2021-04-30, release date: 2021-09-15, Last modification date: 2023-11-29) |
| Primary citation | Obata, J.,Kawakami, N.,Tsutsumi, A.,Nasu, E.,Miyamoto, K.,Kikkawa, M.,Arai, R. Icosahedral 60-meric porous structure of designed supramolecular protein nanoparticle TIP60. Chem.Commun.(Camb.), 57:10226-10229, 2021 Cited by PubMed Abstract: Supramolecular protein nanoparticles and nanocages have potential in a broad range of applications. Recently, we developed a uniform supramolecular protein nanoparticle, TIP60, symmmetrically self-assembled from fusion proteins of a pentameric Sm-like protein and a dimeric MyoX-coil domain. Herein, we report the icosahedral 60-meric structure of TIP60 solved using single-particle cryo-electron microscopy. Interestingly, the structure revealed 20 regular-triangle-like pores on the surface. TIP60 and its mutants have many modifiable sites on their exterior and interior surfaces. The TIP60 architecture will be useful in the development of biomedical and biochemical nanoparticles/nanocages for future applications. PubMed: 34523636DOI: 10.1039/d1cc03114g PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.3 Å) |
Structure validation
Download full validation report
