7EMM

Crystal structure of IrCp* immobilized apo-R52H-rHLFr

Summary for 7EMM

• Entry DOI: 10.2210/pdb7emm/pdb
• Descriptor: Ferritin light chain, 1,2-ETHANEDIOL, SULFATE ION, ... (7 entities in total)
• Functional Keywords: iron storage protein, ir binding, metal binding protein
• Biological source: Equus caballus (Horse)
• Total number of polymer chains: 1
• Total formula weight: 21970.80

Authors

Taher, M.,Maity, B.,Nakane, T.,Abe, S.,Ueno, T.,Mazumdar, S. (deposition date: 2021-04-14, release date: 2022-03-02, Last modification date: 2023-11-29)

Primary citation

Taher, M.,Maity, B.,Nakane, T.,Abe, S.,Ueno, T.,Mazumdar, S.
Controlled Uptake of an Iridium Complex inside Engineered apo-Ferritin Nanocages: Study of Structure and Catalysis.
Angew.Chem.Int.Ed.Engl., 61:e202116623-e202116623, 2022

PubMed Abstract: The effect of the mutation at the core of the ferritin nanocage (apo-rHLFr) on the uptake of IrCp* has been investigated by structural and spectroscopic methods. Site-specific mutations of two polar residues viz., Asp38 and Arg52 were investigated. The uptake of IrCp* was increased by about 1.5-fold on mutation of Arg52 by His compared to the wild-type variant, while mutation of Asp38 by His had no effect on the uptake. All the variants of the Ir-embedded ferritin cages remained as stable as the wild-type analogue. These hybrid bio-nanocages of ferritin were found to efficiently catalyze transfer hydrogenation of various substituted acetophenones forming the corresponding chiral alcohols with up to 88 % conversion and 70 % enantioselectivity. An electron-withdrawing substituent on the reactant enhanced the Turnover frequency of the reaction. Molecular docking analyses suggested that the substrate binds in different orientations at the active site in different mutants of the nanocage.

PubMed: 35005820
DOI: 10.1002/anie.202116623

Experimental method

X-RAY DIFFRACTION (1.25 Å)