7ELY

A peptide with high affinity for B-Cell lymphoma2(Bcl-2)

Summary for 7ELY

• Entry DOI: 10.2210/pdb7ely/pdb
• NMR Information: BMRB: 36419
• Descriptor: 16X_BCL (1 entity in total)
• Functional Keywords: b-cell lymphoma2, disulfide bond, unknown function
• Biological source: Escherichia virus M13
• Total number of polymer chains: 1
• Total formula weight: 2902.43

Authors

Fan, S.,Zha, J.,Wu, C. (deposition date: 2021-04-12, release date: 2021-05-19, Last modification date: 2024-10-16)

Primary citation

Zha, J.,Li, J.,Fan, S.,Duan, Z.,Zhao, Y.,Wu, C.
An evolution-inspired strategy to design disulfide-rich peptides tolerant to extensive sequence manipulation.
Chem Sci, 12:11464-11472, 2021

PubMed Abstract: Natural disulfide-rich peptides (DRPs) are valuable scaffolds for the development of new bioactive molecules and therapeutics. However, there are only a limited number of topologically distinct DRP folds in nature, and most of them suffer from the problem of oxidative folding. Thus, strategies to design DRPs with new constrained topologies beyond the scope of natural folds are desired. Herein we report a general evolution-inspired strategy to design new DRPs with diverse disulfide frameworks, which relies on the incorporation of two cysteine residues and a random peptide sequence into a precursor disulfide-stabilized fold. These peptides can spontaneously fold in redox buffers to the expected tricyclic topologies with high yields. Moreover, we demonstrated that these DRPs can be used as templates for the construction of phage-displayed peptide libraries, enabling the discovery of new DRP ligands from fully randomized sequences. This study thus paves the way for the development of new DRP ligands and therapeutics with structures not derived from natural DRPs.

PubMed: 34567500
DOI: 10.1039/d1sc02952e

Experimental method

SOLUTION NMR