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7EJW

Crystal structure of FleN in complex with FleQ AAA+ doamain

Summary for 7EJW
Entry DOI10.2210/pdb7ejw/pdb
DescriptorTranscriptional antiactivator FleN, Transcriptional regulator FleQ, PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER, ... (6 entities in total)
Functional Keywordsantiactivator, activator, atpase, transcription, fleq, flen
Biological sourcePseudomonas aeruginosa PAO1
More
Total number of polymer chains4
Total formula weight120401.41
Authors
Chanchal,Banerjee, P.,Raghav, S.,Jain, D. (deposition date: 2021-04-02, release date: 2021-12-22, Last modification date: 2024-05-29)
Primary citationBanerjee, P.,Raghav, S.,Goswami, H.N.,Jain, D.
The antiactivator FleN uses an allosteric mechanism to regulate sigma 54 -dependent expression of flagellar genes in Pseudomonas aeruginosa .
Sci Adv, 7:eabj1792-eabj1792, 2021
Cited by
PubMed Abstract: Diverse sigma factors associate with the RNA polymerase (RNAP) core enzyme to initiate transcription of specific target genes in bacteria. σ-Mediated transcription uses AAA+ activators that utilize their ATPase activity for transcription initiation. FleQ is a σ-dependent master transcriptional regulator of flagellar genes in . The ATPase activity of FleQ is regulated via a P-loop ATPase, FleN, through protein-protein interaction. We report a high-resolution crystal structure of the AAA+ domain of FleQ in complex with antiactivator FleN. The data reveal that FleN allosterically prevents ATP binding to FleQ. Furthermore, FleN remodels the region of FleQ essential for engagement with σ for transcription initiation. Disruption of the conserved protein-protein interface, by mutation, shows motility and transcription defects in vivo and multiflagellate phenotype. Our study provides a detailed mechanism used by monoflagellate bacteria to fine-tune the expression of flagellar genes to form and maintain a single flagellum.
PubMed: 34669473
DOI: 10.1126/sciadv.abj1792
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (1.98 Å)
Structure validation

227344

數據於2024-11-13公開中

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