7EJU

Junin virus(JUNV) RNA polymerase L complexed with Z protein

Summary for 7EJU

• Entry DOI: 10.2210/pdb7eju/pdb
• EMDB information: 31163
• Descriptor: RNA-directed RNA polymerase L, RING finger protein Z, ZINC ION, ... (4 entities in total)
• Functional Keywords: junin virus, rna polymerase, l protein, z protein, viral protein
• Biological source: Junin mammarenavirus (Argentinian mammarenavirus, JUNV, Junn mammarenavirus); More
• Total number of polymer chains: 2
• Total formula weight: 264196.68

Authors

Chen, Y. (deposition date: 2021-04-02, release date: 2021-07-07, Last modification date: 2024-10-30)

Primary citation

Kang, H.,Cong, J.,Wang, C.,Ji, W.,Xin, Y.,Qian, Y.,Li, X.,Chen, Y.,Rao, Z.
Structural basis for recognition and regulation of arenavirus polymerase L by Z protein.
Nat Commun, 12:4134-4134, 2021

PubMed Abstract: Junin virus (JUNV) causes Argentine hemorrhagic fever, a debilitating human disease of high mortality rates and a great risk to public health worldwide. Studying the L protein that replicates and transcribes the genome of JUNV, and its regulator Z protein should provide critical clues to identify therapeutic targets for disrupting the life cycle of JUNV. Here we report the 3.54 Å cryo-EM structure of the JUNV L protein complexed with regulator Z protein. JUNV L structure reveals a conserved architecture containing signature motifs found in other L proteins. Structural analysis shows that L protein is regulated by binding of Z protein at the RNA product exit site. Based on these findings, we propose a model for the role of Z protein as a switch to turn on/off the viral RNA synthesis via its interaction with L protein. Our work unveils the mechanism of JUNV transcription, replication and regulation, which provides a framework for the rational design of antivirals for combating viral infections.

PubMed: 34226547
DOI: 10.1038/s41467-021-24458-1

Experimental method

ELECTRON MICROSCOPY (3.5 Å)