7EFO

LptB2FG-LPS from Klebsiella pneumoniae in nanodiscs

Summary for 7EFO

• Entry DOI: 10.2210/pdb7efo/pdb
• EMDB information: 31088
• Descriptor: Lipopolysaccharide export system ATP-binding protein LptB, Lipopolysaccharide export system permease protein LptF, LPS export ABC transporter permease LptG, ... (4 entities in total)
• Functional Keywords: membrane protein, abc transporter
• Biological source: Klebsiella pneumoniae subsp. pneumoniae; More
• Total number of polymer chains: 4
• Total formula weight: 136828.70

Authors

Luo, Q.,Shi, H. (deposition date: 2021-03-22, release date: 2022-03-02, Last modification date: 2025-07-02)

Primary citation

Luo, Q.,Shi, H.,Xu, X.
Cryo-EM structures of LptB 2 FG and LptB 2 FGC from Klebsiella pneumoniae in complex with lipopolysaccharide.
Biochem.Biophys.Res.Commun., 571:20-25, 2021

PubMed Abstract: Lipopolysaccharide (LPS) is an essential component of the outer membrane (OM) in most Gram-negative bacteria. LPS transport from the inner membrane (IM) to the OM is achieved by seven lipopolysaccharide transport proteins (LptA-G). LptBFG, an type VI ATP-binding cassette (ABC) transporter, forms a stable complex with LptC, extracts LPS from the IM and powers LPS transport to the OM. Here we report the cryo-EM structures of LptBFG and LptBFGC from Klebsiella pneumoniae in complex with LPS. The KpLptBFG-LPS structure provides detailed interactions between LPS and the transporter, while the KpLptBFGC-LPS structure may represent an intermediate state that the transmembrane helix of LptC has not been fully inserted into the transmembrane domains of LptBFG.

PubMed: 34303191
DOI: 10.1016/j.bbrc.2021.07.049

Experimental method

ELECTRON MICROSCOPY (3.85 Å)