7EBE

Crystal structure of Isocitrate lyase-1 from Candida albicans

7EBE の概要

• エントリーDOI: 10.2210/pdb7ebe/pdb
• 分子名称: Isocitrate lyase, MAGNESIUM ION, FORMIC ACID, ... (4 entities in total)
• 機能のキーワード: isocitrate lyase, glyoxylate cycle, ubiquitination, lyase
• 由来する生物種: Candida albicans
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 499438.92

構造登録者

Hiragi, K.,Nishio, K.,Moriyama, S.,Hamaguchi, T.,Mizoguchi, A.,Yonekura, K.,Tani, K.,Mizushima, T. (登録日: 2021-03-09, 公開日: 2021-06-23, 最終更新日: 2023-11-29)

主引用文献

Hiragi, K.,Nishio, K.,Moriyama, S.,Hamaguchi, T.,Mizoguchi, A.,Yonekura, K.,Tani, K.,Mizushima, T.
Structural insights into the targeting specificity of ubiquitin ligase for S. cerevisiae isocitrate lyase but not C. albicans isocitrate lyase.
J.Struct.Biol., 213:107748-107748, 2021

PubMed Abstract: In Saccharomyces cerevisiae, the glyoxylate cycle is controlled through the posttranslational regulation of its component enzymes, such as isocitrate lyase (ICL), which catalyzes the first unique step of the cycle. The ICL of S.cerevisiae (ScIcl1) is tagged for proteasomal degradation through ubiquitination by a multisubunit ubiquitin ligase (the glucose-induced degradation-deficient (GID) complex), whereas that of the pathogenic yeast Candida albicans (CaIcl1) escapes this process. However, the reason for the ubiquitin targeting specificity of the GID complex for ScIcl1 and not for CaIcl1 is unclear. To gain some insight into this, in this study, the crystal structures of apo ScIcl1 and CaIcl1 in complex with formate and the cryogenic electron microscopy structure of apo CaIcl1 were determined at a resolution of 2.3, 2.7, and 2.6 Å, respectively. A comparison of the various structures suggests that the orientation of N-terminal helix α1 in S.cerevisiae is likely key to repositioning of ubiquitination sites and contributes to the distinction found in C. albicans ubiquitin evasion mechanism. This finding gives us a better understanding of the molecular mechanism of ubiquitin-dependent ScIcl1 degradation and could serve as a theoretical basis for the research and development of anti-C. albicans drugs based on the concept of CaIcl1 ubiquitination.

PubMed: 34033899
DOI: 10.1016/j.jsb.2021.107748

実験手法

X-RAY DIFFRACTION (2.69 Å)