7E3K

Ultrapotent SARS-CoV-2 neutralizing antibodies with protective efficacy against newly emerged mutational variants

Summary for 7E3K

• Entry DOI: 10.2210/pdb7e3k/pdb
• EMDB information: 30982 30983
• Descriptor: Spike glycoprotein, 13G9 heavy chain, 13G9 light chain, ... (4 entities in total)
• Functional Keywords: covid-19, spike glycoprotein, virus, viral protein, antibody
• Biological source: Severe acute respiratory syndrome coronavirus 2 (2019-nCoV); More
• Total number of polymer chains: 9
• Total formula weight: 573276.21

Authors

Guo, H.,Li, T.,Liu, F.,Gao, Y.,Ji, X.,Yang, H. (deposition date: 2021-02-09, release date: 2021-09-15, Last modification date: 2024-11-06)

Primary citation

Li, T.,Han, X.,Gu, C.,Guo, H.,Zhang, H.,Wang, Y.,Hu, C.,Wang, K.,Liu, F.,Luo, F.,Zhang, Y.,Hu, J.,Wang, W.,Li, S.,Hao, Y.,Shen, M.,Huang, J.,Long, Y.,Song, S.,Wu, R.,Mu, S.,Chen, Q.,Gao, F.,Wang, J.,Long, S.,Li, L.,Wu, Y.,Gao, Y.,Xu, W.,Cai, X.,Qu, D.,Zhang, Z.,Zhang, H.,Li, N.,Gao, Q.,Zhang, G.,He, C.,Wang, W.,Ji, X.,Tang, N.,Yuan, Z.,Xie, Y.,Yang, H.,Zhang, B.,Huang, A.,Jin, A.
Potent SARS-CoV-2 neutralizing antibodies with protective efficacy against newly emerged mutational variants.
Nat Commun, 12:6304-6304, 2021

PubMed Abstract: Accumulating mutations in the SARS-CoV-2 Spike (S) protein can increase the possibility of immune escape, challenging the present COVID-19 prophylaxis and clinical interventions. Here, 3 receptor binding domain (RBD) specific monoclonal antibodies (mAbs), 58G6, 510A5 and 13G9, with high neutralizing potency blocking authentic SARS-CoV-2 virus display remarkable efficacy against authentic B.1.351 virus. Surprisingly, structural analysis has revealed that 58G6 and 13G9 both recognize the steric region S on the RBD, overlapping the E484K mutation presented in B.1.351. Also, 58G6 directly binds to another region S in the RBD. Significantly, 58G6 and 510A5 both demonstrate prophylactic efficacy against authentic SARS-CoV-2 and B.1.351 viruses in the transgenic mice expressing human ACE2 (hACE2), protecting weight loss and reducing virus loads. Together, we have evidenced 2 potent neutralizing Abs with unique mechanism targeting authentic SARS-CoV-2 mutants, which can be promising candidates to fulfill the urgent needs for the prolonged COVID-19 pandemic.

PubMed: 34728625
DOI: 10.1038/s41467-021-26539-7

Experimental method

ELECTRON MICROSCOPY (3.9 Å)