7DVQ
Cryo-EM Structure of the Activated Human Minor Spliceosome (minor Bact Complex)
Summary for 7DVQ
| Entry DOI | 10.2210/pdb7dvq/pdb |
| EMDB information | 30875 |
| Descriptor | Pre-mRNA-processing-splicing factor 8, Small nuclear ribonucleoprotein F, Small nuclear ribonucleoprotein E, ... (49 entities in total) |
| Functional Keywords | spliceosome, minor spliceosome, bact complex, human spliceosome, ppil2, scnm1, rbm48-armc7 complex, u12-type intron, u6atac snrna, splicing |
| Biological source | Homo sapiens (Human) More |
| Total number of polymer chains | 49 |
| Total formula weight | 2918375.89 |
| Authors | |
| Primary citation | Bai, R.,Wan, R.,Wang, L.,Xu, K.,Zhang, Q.,Lei, J.,Shi, Y. Structure of the activated human minor spliceosome. Science, 371:-, 2021 Cited by PubMed Abstract: The minor spliceosome mediates splicing of the rare but essential U12-type precursor messenger RNA. Here, we report the atomic features of the activated human minor spliceosome determined by cryo-electron microscopy at 2.9-angstrom resolution. The 5' splice site and branch point sequence of the U12-type intron are recognized by the U6atac and U12 small nuclear RNAs (snRNAs), respectively. Five newly identified proteins stabilize the conformation of the catalytic center: The zinc finger protein SCNM1 functionally mimics the SF3a complex of the major spliceosome, the RBM48-ARMC7 complex binds the γ-monomethyl phosphate cap at the 5' end of U6atac snRNA, the U-box protein PPIL2 coordinates loop I of U5 snRNA and stabilizes U5 small nuclear ribonucleoprotein (snRNP), and CRIPT stabilizes U12 snRNP. Our study provides a framework for the mechanistic understanding of the function of the human minor spliceosome. PubMed: 33509932DOI: 10.1126/science.abg0879 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (2.89 Å) |
Structure validation
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