7DVQ

Cryo-EM Structure of the Activated Human Minor Spliceosome (minor Bact Complex)

7DVQ の概要

• エントリーDOI: 10.2210/pdb7dvq/pdb
• EMDBエントリー: 30875
• 分子名称: Pre-mRNA-processing-splicing factor 8, Small nuclear ribonucleoprotein F, Small nuclear ribonucleoprotein E, ... (49 entities in total)
• 機能のキーワード: spliceosome, minor spliceosome, bact complex, human spliceosome, ppil2, scnm1, rbm48-armc7 complex, u12-type intron, u6atac snrna, splicing
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 49
• 化学式量合計: 2918375.89

構造登録者

Bai, R.,Wan, R.,Wang, L.,Xu, K.,Zhang, Q.,Lei, J.,Shi, Y. (登録日: 2021-01-14, 公開日: 2021-03-31, 最終更新日: 2024-11-13)

主引用文献

Bai, R.,Wan, R.,Wang, L.,Xu, K.,Zhang, Q.,Lei, J.,Shi, Y.
Structure of the activated human minor spliceosome.
Science, 371:-, 2021

PubMed Abstract: The minor spliceosome mediates splicing of the rare but essential U12-type precursor messenger RNA. Here, we report the atomic features of the activated human minor spliceosome determined by cryo-electron microscopy at 2.9-angstrom resolution. The 5' splice site and branch point sequence of the U12-type intron are recognized by the U6atac and U12 small nuclear RNAs (snRNAs), respectively. Five newly identified proteins stabilize the conformation of the catalytic center: The zinc finger protein SCNM1 functionally mimics the SF3a complex of the major spliceosome, the RBM48-ARMC7 complex binds the γ-monomethyl phosphate cap at the 5' end of U6atac snRNA, the U-box protein PPIL2 coordinates loop I of U5 snRNA and stabilizes U5 small nuclear ribonucleoprotein (snRNP), and CRIPT stabilizes U12 snRNP. Our study provides a framework for the mechanistic understanding of the function of the human minor spliceosome.

PubMed: 33509932
DOI: 10.1126/science.abg0879

実験手法

ELECTRON MICROSCOPY (2.89 Å)