7DI8

Electron crystallographic structure of Catalase using a direct electron detector at 300 kV

Summary for 7DI8

• Entry DOI: 10.2210/pdb7di8/pdb
• Descriptor: Catalase, PROTOPORPHYRIN IX CONTAINING FE, NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE (3 entities in total)
• Functional Keywords: electron 3d crystallography, direct detector, cryo arm, parallem, oxidoreductase
• Biological source: Bos taurus (Bovine)
• Total number of polymer chains: 4
• Total formula weight: 245444.27

Authors

Takaba, K.,Maki-Yonekura, S.,Yonekura, K. (deposition date: 2020-11-18, release date: 2020-12-09, Last modification date: 2024-03-27)

Primary citation

Takaba, K.,Maki-Yonekura, S.,Inoue, S.,Hasegawa, T.,Yonekura, K.
Protein and Organic-Molecular Crystallography With 300kV Electrons on a Direct Electron Detector.
Front Mol Biosci, 7:612226-612226, 2020

PubMed Abstract: Electron 3D crystallography can reveal the atomic structure from undersized crystals of various samples owing to the strong scattering power of electrons. Here, a direct electron detector DE64 was tested for small and thin crystals of protein and an organic molecule using a JEOL CRYO ARM 300 electron microscope. The microscope is equipped with a cold-field emission gun operated at an accelerating voltage of 300 kV, quad condenser lenses for parallel illumination, an in-column energy filter, and a stable rotational goniometer stage. Rotational diffraction data were collected in an unsupervised manner from crystals of a heme-binding enzyme catalase and a representative organic semiconductor material Ph-BTBT-C10. The structures were determined by molecular replacement for catalase and by the direct method for Ph-BTBT-C10. The analyses demonstrate that the system works well for electron 3D crystallography of these molecules with less damaging, a smaller point spread, and less noise than using the conventional scintillator-coupled camera.

PubMed: 33469549
DOI: 10.3389/fmolb.2020.612226

Experimental method

ELECTRON CRYSTALLOGRAPHY (3.2 Å)