7DF4

SARS-CoV-2 S-ACE2 complex

Summary for 7DF4

• Entry DOI: 10.2210/pdb7df4/pdb
• EMDB information: 30661
• Descriptor: Angiotensin-converting enzyme 2, Spike glycoprotein, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (4 entities in total)
• Functional Keywords: spike glycoprotein, coronavirus, sars-cov-2 virus, receptor ace2, viral protein
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 4
• Total formula weight: 508166.92

Authors

Cong, X.,Yao, C. (deposition date: 2020-11-06, release date: 2020-12-16, Last modification date: 2024-10-23)

Primary citation

Xu, C.,Wang, Y.,Liu, C.,Zhang, C.,Han, W.,Hong, X.,Wang, Y.,Hong, Q.,Wang, S.,Zhao, Q.,Wang, Y.,Yang, Y.,Chen, K.,Zheng, W.,Kong, L.,Wang, F.,Zuo, Q.,Huang, Z.,Cong, Y.
Conformational dynamics of SARS-CoV-2 trimeric spike glycoprotein in complex with receptor ACE2 revealed by cryo-EM.
Sci Adv, 7:-, 2021

PubMed Abstract: The recent outbreaks of SARS-CoV-2 pose a global health emergency. The SARS-CoV-2 trimeric spike (S) glycoprotein interacts with the human ACE2 receptor to mediate viral entry into host cells. We report the cryo-EM structures of a tightly closed SARS-CoV-2 S trimer with packed fusion peptide and an ACE2-bound S trimer at 2.7- and 3.8-Å resolution, respectively. Accompanying ACE2 binding to the up receptor-binding domain (RBD), the associated ACE2-RBD exhibits continuous swing motions. Notably, the SARS-CoV-2 S trimer appears much more sensitive to the ACE2 receptor than the SARS-CoV S trimer regarding receptor-triggered transformation from the closed prefusion state to the fusion-prone open state, potentially contributing to the superior infectivity of SARS-CoV-2. We defined the RBD T470-T478 loop and Y505 as viral determinants for specific recognition of SARS-CoV-2 RBD by ACE2. Our findings depict the mechanism of ACE2-induced S trimer conformational transitions from the ground prefusion state toward the postfusion state, facilitating development of anti-SARS-CoV-2 vaccines and therapeutics.

PubMed: 33277323
DOI: 10.1126/sciadv.abe5575

Experimental method

ELECTRON MICROSCOPY (3.8 Å)