7DDZ

The Crystal Structure of Human Neuropeptide Y Y2 Receptor with JNJ-31020028

7DDZ の概要

• エントリーDOI: 10.2210/pdb7ddz/pdb
• 分子名称: Human Neuropeptide Y Y2 Receptor fusion protein, ~{N}-[4-[4-[(1~{S})-2-(diethylamino)-2-oxidanylidene-1-phenyl-ethyl]piperazin-1-yl]-3-fluoranyl-phenyl]-2-pyridin-3-yl-benzamide, FLAVIN MONONUCLEOTIDE (3 entities in total)
• 機能のキーワード: neuropeptide y y2 receptor, membrane protein
• 由来する生物種: Enterobacteria phage RB59; 詳細
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 75307.32

構造登録者

Tang, T.,Han, S.,Zhao, Q.,Wu, B. (登録日: 2020-10-30, 公開日: 2021-01-27, 最終更新日: 2024-10-16)

主引用文献

Tang, T.,Hartig, C.,Chen, Q.,Zhao, W.,Kaiser, A.,Zhang, X.,Zhang, H.,Qu, H.,Yi, C.,Ma, L.,Han, S.,Zhao, Q.,Beck-Sickinger, A.G.,Wu, B.
Structural basis for ligand recognition of the neuropeptide Y Y 2 receptor.
Nat Commun, 12:737-737, 2021

PubMed Abstract: The human neuropeptide Y (NPY) Y receptor (YR) plays essential roles in food intake, bone formation and mood regulation, and has been considered an important drug target for obesity and anxiety. However, development of drugs targeting YR remains challenging with no success in clinical application yet. Here, we report the crystal structure of YR bound to a selective antagonist JNJ-31020028 at 2.8 Å resolution. The structure reveals molecular details of the ligand-binding mode of YR. Combined with mutagenesis studies, the YR structure provides insights into key factors that define antagonistic activity of diverse antagonists. Comparison with the previously determined antagonist-bound YR structures identified receptor-ligand interactions that play different roles in modulating receptor activation and mediating ligand selectivity. These findings deepen our understanding about molecular mechanisms of ligand recognition and subtype specificity of NPY receptors, and would enable structure-based drug design.

PubMed: 33531491
DOI: 10.1038/s41467-021-21030-9

実験手法

X-RAY DIFFRACTION (2.8 Å)