7DDX

Crystal structure of KANK1 S1179F mutant in complex wtih eIF4A1

7DDX の概要

• エントリーDOI: 10.2210/pdb7ddx/pdb
• 分子名称: KN motif and ankyrin repeat domains 1, Eukaryotic initiation factor 4A-I, SULFATE ION, ... (5 entities in total)
• 機能のキーワード: nephrotic syndrome, kidney ankyrin repeat-containing protein, eukaryotic initiation factor, complex, protein binding
• 由来する生物種: Mus musculus (Mouse); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 55147.86

構造登録者

Pan, W.,Xu, Y.,Wei, Z. (登録日: 2020-10-30, 公開日: 2021-09-08, 最終更新日: 2023-11-29)

主引用文献

Xu, Y.,Guo, C.,Pan, W.,Zhao, C.,Ding, Y.,Xie, X.,Wei, Z.,Sun, Y.,Yu, C.
Nephrotic-syndrome-associated mutation of KANK2 induces pathologic binding competition with physiological interactor KIF21A.
J.Biol.Chem., 297:100958-100958, 2021

PubMed Abstract: Nephrotic syndrome (NS) is a common kidney disorder caused by dysfunction of the glomerular filtration barrier. Some genetic mutations identified in NS patients cause amino acid substitutions of kidney ankyrin repeat-containing (KANK) proteins, which are scaffold proteins that regulate actin polymerization, microtubule targeting, and cell adhesion via binding to various molecules, including the kinesin motor protein KIF21A. However, the mechanisms by which these mutations lead to NS are unclear. Here, we unexpectedly found that the eukaryotic translation initiation factor 4A1 (eIF4A1) interacts with an NS-associated KANK2 mutant (S684F) but not the wild-type protein. Biochemical and structural analyses revealed that the pathological mutation induces abnormal binding of eIF4A1 to KANK2 at the physiological KIF21A-binding site. Competitive binding assays further indicated that eIF4A1 can compete with KIF21A to interact with the S684F mutant of KANK2. In cultured mouse podocytes, this S684F mutant interfered with the KANK2/KIF21A interaction by binding to eIF4A1, and failed to rescue the focal adhesion or cell adhesion that had been reduced or morphologically changed by KANK2 knockout. These structural, biochemical, and cellular results not only provide mechanistic explanations for the podocyte defects caused by the S684F mutation, but also show how a gain-of-binding mutation can lead to a loss-of-function effect.

PubMed: 34274317
DOI: 10.1016/j.jbc.2021.100958

実験手法

X-RAY DIFFRACTION (2.5 Å)