7DBH
The mouse nucleosome structure containing H3mm18
Summary for 7DBH
| Entry DOI | 10.2210/pdb7dbh/pdb |
| Related | 7VBM |
| EMDB information | 30631 31882 |
| Descriptor | Histone H3mm18, Histone H4, Histone H2A type 1-B, ... (6 entities in total) |
| Functional Keywords | complex, chromatin, nucleosome, dna binding protein, dna binding protein-dna complex |
| Biological source | Mus musculus More |
| Total number of polymer chains | 10 |
| Total formula weight | 201077.90 |
| Authors | Hirai, S.,Takizawa, Y.,Kujirai, T.,Kurumizaka, H. (deposition date: 2020-10-20, release date: 2022-01-19, Last modification date: 2024-05-29) |
| Primary citation | Hirai, S.,Tomimatsu, K.,Miyawaki-Kuwakado, A.,Takizawa, Y.,Komatsu, T.,Tachibana, T.,Fukushima, Y.,Takeda, Y.,Negishi, L.,Kujirai, T.,Koyama, M.,Ohkawa, Y.,Kurumizaka, H. Unusual nucleosome formation and transcriptome influence by the histone H3mm18 variant. Nucleic Acids Res., 50:72-91, 2022 Cited by PubMed Abstract: Histone H3mm18 is a non-allelic H3 variant expressed in skeletal muscle and brain in mice. However, its function has remained enigmatic. We found that H3mm18 is incorporated into chromatin in cells with low efficiency, as compared to H3.3. We determined the structures of the nucleosome core particle (NCP) containing H3mm18 by cryo-electron microscopy, which revealed that the entry/exit DNA regions are drastically disordered in the H3mm18 NCP. Consistently, the H3mm18 NCP is substantially unstable in vitro. The forced expression of H3mm18 in mouse myoblast C2C12 cells markedly suppressed muscle differentiation. A transcriptome analysis revealed that the forced expression of H3mm18 affected the expression of multiple genes, and suppressed a group of genes involved in muscle development. These results suggest a novel gene expression regulation system in which the chromatin landscape is altered by the formation of unusual nucleosomes with a histone variant, H3mm18, and provide important insight into understanding transcription regulation by chromatin. PubMed: 34929737DOI: 10.1093/nar/gkab1137 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.6 Å) |
Structure validation
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