7D9V

CRTC1 pSer245 peptide in complex with 14-3-3 zeta

Summary for 7D9V

• Entry DOI: 10.2210/pdb7d9v/pdb
• Descriptor: 14-3-3 protein zeta/delta, CRTC1 pSer245 peptide, GLYCEROL, ... (4 entities in total)
• Functional Keywords: 14-3-3, crtc, protein binding
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 4
• Total formula weight: 62557.50

Authors

Chen, H.,Zhang, H.,Xiang, S. (deposition date: 2020-10-14, release date: 2021-02-24, Last modification date: 2024-10-09)

Primary citation

Chen, H.,Zhang, H.,Chen, P.,Xiang, S.
Structural Insights into the Interaction Between CRTCs and 14-3-3.
J.Mol.Biol., 433:166874-166874, 2021

PubMed Abstract: The CREB-Regulated Transcriptional Coactivators (CRTCs) regulate the transcription of CREB target genes and have important functions in many biological processes. At the basal state, they are phosphorylated at multiple residues, which promotes their association with 14-3-3 that sequesters them in the cytoplasm. Upon dephosphorylation, they translocate into the nuclei and associate with CREB to activate the target gene transcription. Although three conserved serine residues in CRTCs have been implicated in their phosphorylation regulation, whether and how they mediate interactions with 14-3-3 is unclear. Here, we provide direct evidence that these residues and flanking regions interact with 14-3-3 and the structural basis of the interaction. Our study also identified a novel salt bridge in CRTC1 with an important function in binding 14-3-3, expanding the understanding of the interaction between 14-3-3 and its ligands.

PubMed: 33556406
DOI: 10.1016/j.jmb.2021.166874

Experimental method

X-RAY DIFFRACTION (2.21 Å)