7D3K

FOOT AND MOUTH DISEASE VIRUS O/TIBET/99-BOUND THE SINGLE CHAIN FRAGMEN ANTIBODY B77

Summary for 7D3K

• Entry DOI: 10.2210/pdb7d3k/pdb
• EMDB information: 30558 3631
• Descriptor: O/TIBET/99 VP1, O/TIBET/99 VP2, O/TIBET/99 VP3, ... (6 entities in total)
• Functional Keywords: foot and mouth disease virus, fmdv, virus
• Biological source: Bos taurus; More
• Total number of polymer chains: 6
• Total formula weight: 106791.08

Authors

He, Y.,Lou, Z. (deposition date: 2020-09-19, release date: 2021-04-14, Last modification date: 2024-03-27)

Primary citation

He, Y.,Li, K.,Cao, Y.,Sun, Z.,Li, P.,Bao, H.,Wang, S.,Zhu, G.,Bai, X.,Sun, P.,Liu, X.,Yang, C.,Liu, Z.,Lu, Z.,Rao, Z.,Lou, Z.
Structures of Foot-and-mouth Disease Virus with neutralizing antibodies derived from recovered natural host reveal a mechanism for cross-serotype neutralization.
Plos Pathog., 17:e1009507-e1009507, 2021

PubMed Abstract: The development of a universal vaccine against foot-and-mouth disease virus (FMDV) is hindered by cross-serotype antigenic diversity and by a lack of knowledge regarding neutralization of the virus in natural hosts. In this study, we isolated serotype O-specific neutralizing antibodies (NAbs) (F145 and B77) from recovered natural bovine hosts by using the single B cell antibody isolation technique. We also identified a serotype O/A cross-reacting NAb (R50) and determined virus-NAb complex structures by cryo-electron microscopy at near-atomic resolution. F145 and B77 were shown to engage the capsid of FMDV-O near the icosahedral threefold axis, binding to the BC/HI-loop of VP2. In contrast, R50 engages the capsids of both FMDV-O and FMDV-A between the 2- and 5-fold axes and binds to the BC/EF/GH-loop of VP1 and to the GH-loop of VP3 from two adjacent protomers, revealing a previously unknown antigenic site. The cross-serotype neutralizing epitope recognized by R50 is highly conserved among serotype O/A. These findings help to elucidate FMDV neutralization by natural hosts and provide epitope information for the development of a universal vaccine for cross-serotype protection against FMDV.

PubMed: 33909694
DOI: 10.1371/journal.ppat.1009507

Experimental method

ELECTRON MICROSCOPY (3.9 Å)